Phosphorylation of cold shock domain/Y‐box proteins by ERK2 and GSK3β and repression of the human VEGF promoter
- 20 September 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (24), 5372-5378
- https://doi.org/10.1016/j.febslet.2005.08.075
Abstract
The hypoxia responsive region (HRR) of the VEGF promoter plays a key role in regulating VEGF expression. We found that the cold shock domain (Y-box) repressor proteins, dbpA and dbpB/YB-1, bind distinct strands of the human VEGF HRR. We find both dbpA and dbpB are phosphorylated by ERK2 and GSK3beta in vitro, and the binding of dbpB to single-strand VEGF HRR DNA is regulated by this phosphorylation. These findings suggest the ERK/MAPK and PI3K pathways may regulate VEGF expression in part through regulating the action of these repressor proteins.Keywords
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