In vitro suppression of serum elastase-inhibitory capacity by reactive oxygen species generated by phagocytosing polymorphonuclear leukocytes.
Open Access
- 1 April 1979
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 63 (4), 793-797
- https://doi.org/10.1172/jci109364
Abstract
Human polymorphonuclear leukocytes (PMN) phagocytosing opsonized antigen-antibody complexes, produce dialyzable species of activated oxygen which are capable of partially suppressing the elastase-inhibiting capacity (EIC) of whole human serum or purified human alpha1-proteinase inhibitor. Serum EIC was partially protially protected by superoxide dismutase, catalase, or mannitol, suggesting that hydroxyl radical, formed by interaction of superoxide radical and hydrogen peroxide, might be responsible for this effect. NaN3 also partly protected EIC, implicating myeloperoxidase-mediated reactions as well. An artificial superoxide rradical-generating system, involving xanthine and xanthine-oxidase, could be substituted for phagocytosing PMN with resultant EIC suppression. These results are consistent with previous demonstrations of the release of potent oxidants by stimulated PMN, as well as earlier studies from our laboratory showing sensitivity of alpha1-proteinase inhibitor to inactivation by oxidants. Oxidative inactivation of proteinase inhibitors in the microenvironment of PMN accumulating at sites of inflammation may allow proteases released from these cells to more readily damage adjacent connective tissue structures.This publication has 11 references indexed in Scilit:
- Human granulocyte generation of hydroxyl radical.The Journal of Experimental Medicine, 1978
- Superoxide‐dependent production of hydroxyl radical catalyzed by iron—EDTA complexFEBS Letters, 1978
- POSSIBLE MECHANISMS OF EMPHYSEMA IN SMOKERS - INVITRO SUPPRESSION OF SERUM ELASTASE INHIBITORY CAPACITY BY FRESH CIGARETTE-SMOKE AND ITS PREVENTION BY ANTIOXIDANTSPublished by Elsevier BV ,1978
- The role of lysosomal elastase in the digestion of Escherichia coli proteins by human polymorphonuclear leukocytes: experiments with living leukocytes.JCI Insight, 1976
- Free radicals and inflammation. Protection of phagocytosine leukocytes by superoxide dismutase.JCI Insight, 1975
- The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes.JCI Insight, 1975
- Free Radicals and Inflammation: Protection of Synovial Fluid by Superoxide DismutaseScience, 1974
- Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).1969
- MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMESThe Journal of Experimental Medicine, 1968
- A SPECTROPHOTOMETRIC METHOD FOR MEASURING THE BREAKDOWN OF HYDROGEN PEROXIDE BY CATALASEJournal of Biological Chemistry, 1952