Historical perspectives in fat cell biology: the fat cell as a model for the investigation of hormonal and metabolic pathways
- 15 January 2012
- journal article
- review article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 302 (2), C327-C359
- https://doi.org/10.1152/ajpcell.00168.2011
Abstract
For many years, there was little interest in the biochemistry or physiology of adipose tissue. It is now well recognized that adipocytes play an important dynamic role in metabolic regulation. They are able to sense metabolic states via their ability to perceive a large number of nervous and hormonal signals. They are also able to produce hormones, called adipokines, that affect nutrient intake, metabolism and energy expenditure. The report by Rodbell in 1964 that intact fat cells can be obtained by collagenase digestion of adipose tissue revolutionized studies on the hormonal regulation and metabolism of the fat cell. In the context of the advent of systems biology in the field of cell biology, the present seems an appropriate time to look back at the global contribution of the fat cell to cell biology knowledge. This review focuses on the very early approaches that used the fat cell as a tool to discover and understand various cellular mechanisms. Attention essentially focuses on the early investigations revealing the major contribution of mature fat cells and also fat cells originating from adipose cell lines to the discovery of major events related to hormone action (hormone receptors and transduction pathways involved in hormonal signaling) and mechanisms involved in metabolite processing (hexose uptake and uptake, storage, and efflux of fatty acids). Dormant preadipocytes exist in the stroma-vascular fraction of the adipose tissue of rodents and humans; cell culture systems have proven to be valuable models for the study of the processes involved in the formation of new fat cells. Finally, more recent insights into adipocyte secretion, a completely new role with major metabolic impact, are also briefly summarized.Keywords
This publication has 88 references indexed in Scilit:
- Lipolysis – A highly regulated multi-enzyme complex mediates the catabolism of cellular fat storesProgress in Lipid Research, 2011
- Endoplasmic Reticulum Stress and the Inflammatory Basis of Metabolic DiseaseCell, 2010
- Cellular fatty acid uptake: a pathway under constructionTrends in Endocrinology & Metabolism, 2009
- Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined FactorsCell, 2007
- Co-culture with fat cells induces cellular insulin resistance in primary hepatocytesBiochemical and Biophysical Research Communications, 2006
- Surface protein characterization of human adipose tissue‐derived stromal cellsJournal of Cellular Physiology, 2001
- Caveolin, a protein component of caveolae membrane coatsCell, 1992
- Stereochemical aspects of β-adrenoceptor antagonist-receptor interaction in adipocytes. Differentiation of β-adrenoceptors in human and rat adipocytesLife Sciences, 1976
- The influence of pituitary hormones and norepinephrine on the size of adipose cells in organ cultureThe Anatomical Record, 1969
- The effect of proteolytic enzymes on isolated adipose cellsLife Sciences, 1966