NGF‐dependent and NGF‐independent recovery of sympathetic function after chemical sympathectomy with 6‐hydroxydopamine
- 4 September 1995
- journal article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 359 (4), 586-594
- https://doi.org/10.1002/cne.903590406
Abstract
To help clarify the distinction between the nerve growth factor (NGF)‐dependent collateral sprouting of sympathetic nerves and their NGF‐independent regeneration after crush, we used 6‐hydoxydopamine (OHDA) to destroy the sympathetic terminals in adult rats; this leaves the axons damage‐free. Would recovery occur by regeneration and/or collateral sprouting? A single 6‐OHDA injection abolished the sympathetic pilomotor field revealed by electrical stimulation of a cutaneous nerve. Recovery began within 2 days, and by 20 days the field was reestablished. If the field was “isolated” by adjacent denervations at the time of 6‐OHDA treatment, the recovering pilomotor field expanded extensively into the surrounding territory. In the presence of anti‐NGF, however, the pilomotor field expansion ceased at about 60% of its former size; if anti‐NGF treatment was discontinued, expansion recommenced and extended into the surrounding skin. We suggest that the latter, NGF‐dependent, growth phase corresponds to collateral sprouting, and the initial NGF‐independent one to regeneration. After simple nerve crush, however, such regeneration can triple the normal sympathetic field size. This difference between crush‐ and 6‐OHDA‐induced regeneration might relate to the “cell body reaction” (CBR); the CBR is reduced with increasing distance of the lesion and is undetectable after a 6‐OHDA lesion. Since the CBR and the vigor of regeneration are both increased by repeated axonal injury, we tested the effects of multiple 6‐OHDA treatments; this significantly increased the initial NGF‐independent expansion. We hyprthesize that regeneration is regulated largely by mechanisms associated with the CBR, and that neurotrophin‐dependent collateral sprouting occurs independently of these.Keywords
This publication has 51 references indexed in Scilit:
- Sympathetic nerves in adult rats regenerate normally and restore pilomotor function during an anti‐NGF treatment that prevents their collateral sproutingJournal of Comparative Neurology, 1992
- The blood–nerve barrier in Wallerian degeneration: A sequential long‐term studyMuscle & Nerve, 1989
- Brain-derived neurotrophic factor prevents neuronal death in vivoNature, 1988
- Nerve growth factor-independent development of embryonic mouse sympathetic neurons in dissociated cell cultureDevelopmental Biology, 1985
- Spontaneous regeneration of cut axons in adult rat brainJournal of Comparative Neurology, 1982
- Somatic and behavioural postnatal effects of fetal injections of nerve growth factor antibodies in the ratNature, 1981
- Regeneration of adrenergic axons in rat sciatic nerve: Effect of a conditioning lesionBrain Research, 1978
- A quantitative bioassay for nerve growth factor (NGF) activity employing a clonal pheochromocytoma cell lineBrain Research, 1977
- Axonal regeneration in the rat sciatic nerve: Effect of a conditioning lesion and of dbcAMPBrain Research, 1977
- Catecholamines in peripheral tissues of mice and cell counts of sympathetic ganglia after the prenatal and postnatal administration of the nerve growth factor antiserumInternational Journal of Neuropharmacology, 1967