Carbohydrate–energy restriction may protect the rat brain against oxidative damage and improve physical performance
Open Access
- 1 January 2003
- journal article
- Published by Cambridge University Press (CUP) in British Journal of Nutrition
- Vol. 89 (1), 89-96
- https://doi.org/10.1079/bjn2002749
Abstract
Chronic energy restriction, α-tocopherol supplementation and their interaction with exhaustive exercise were investigated. Eleven-week-old male Wistar rats (n 6×10) were fed either a control (C), a 30 % carbohydrate-energy-restricted control (R) or an α-tocopherol-supplemented (S) diet for 5 months. The animals in each diet were divided into exercised (E) and non-exercised (NE) groups. Before killing, the exercised rats were required to run to exhaustion (39 (SE 6), 69 (se 11) and 18 (se 2) min for the C, R and S groups, respectively). Lipid peroxidation (thiobarbituric acid-reactive substances; TBARS), protein damage (reactive carbonyls) and α-tocopherol were determined in gastrocnemius, liver, brain an/r plasma. There was no difference in lipid peroxidation between the R and C groups, but in liver and muscle peroxidation appeared significantly lower in the S than the other two diets. TBARS in the brain were similar in all groups. On the other hand, reactive carbonyls showed that both the R and S diets reduced protein damage in the brain, while exhaustive exercise increased it. For liver and muscle, however, reactive carbonyl levels were similar in all groups. α-Tocopherol supplementation increased the vitamin concentrations in liver, muscle and plasma, but exercise decreased them in plasma and brain. Carbohydrate-energy restriction increased (P=0·0025) resistance to exhaustive exercise considerably without depleting stores of α-tocopherol or exacerbating oxidative damage in monitored tissues. It is concluded that while exhaustive exercise promotes a tissue-specific oxidative damage detectable only in brain proteins, both experimental diets tended to ameliorate this condition.Keywords
This publication has 43 references indexed in Scilit:
- Alterações metabólicas induzidas pela restrição energética e pela suplementação com vitamina E em ratos submetidos ao exercícioRevista de Nutrição, 2002
- Effects of exhaustive exercise and vitamin b6 deficiency on free radical oxidative process in male trained ratsFree Radical Biology & Medicine, 1996
- Effect of Age and Caloric Intake on Protein Oxidation in Different Brain Regions and on Behavioral Functions of the MouseArchives of Biochemistry and Biophysics, 1996
- Caloric Restriction and AgingScientific American, 1996
- Role of protein oxidation in aging and in age-associated neurodegenerative diseasesLife Sciences, 1994
- Vitamin E Delays Diabetes Onset in the Non-Obese Diabetic MouseHormone and Metabolic Research, 1994
- Increased susceptibility of cellular membranes to the induction of oxidative stress after ingestion of high doses of fish oil: effect of aging and protective action of dl-α tocopherol supplementationThe Journal of Nutritional Biochemistry, 1993
- Vitamin E inhibits protein oxidation in skeletal muscle of resting and exercised ratsBiochemical and Biophysical Research Communications, 1992
- Oxidative DNA damage levels in rats fed low-fat, high-fat, or calorie-restricted dietsToxicology and Applied Pharmacology, 1992
- Retardation of the Aging Processes in Rats by Food RestrictionAnnals of the New York Academy of Sciences, 1991