P70 S6 kinase mediates tau phosphorylation and synthesis
Open Access
- 6 December 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (1), 107-114
- https://doi.org/10.1016/j.febslet.2005.11.059
Abstract
Currently, we found that the 70‐kDa p70 S6 kinase (p70S6K) directly phosphorylates tau at S262, S214, and T212 sites in vitro. By immunoprecipitation, p‐p70S6K (T421/S424) showed a close association with p‐tau (S262 and S396/404). Zinc‐induced p70S6K activation could only upregulate translation of total S6 and tau but not global proteins in SH‐SY5Y cells. The requirement of p70S6K activation was confirmed in the SH‐SY5Y cells that overexpress wild‐type htau40. Level of p‐p70S6K (T421/S424) was only significantly correlated with p‐tau at S262, S214, and T212, but not T212/S214, in Alzheimer's disease (AD) brains. These suggested that p70S6K might contribute to tau related pathologies in AD brains.Keywords
This publication has 53 references indexed in Scilit:
- Expression of tau mRNA and soluble tau isoforms in affected and non‐affected brain areas in Alzheimer's diseaseFEBS Letters, 2004
- Up-Regulation of Phosphorylated/Activated p70 S6 Kinase and Its Relationship to Neurofibrillary Pathology in Alzheimer's DiseaseThe American Journal of Pathology, 2003
- Phosphoprotein Analysis Using Antibodies Broadly Reactive against Phosphorylated MotifsJournal of Biological Chemistry, 2002
- Extracellular Zinc Activates p70 S6 Kinase through the Phosphatidylinositol 3-Kinase Signaling PathwayJournal of Biological Chemistry, 2000
- α-Synuclein Binds to Tau and Stimulates the Protein Kinase A-catalyzed Tau Phosphorylation of Serine Residues 262 and 356Journal of Biological Chemistry, 1999
- Activation of Phospholipase C-γ by the Concerted Action of Tau Proteins and Arachidonic AcidPublished by Elsevier BV ,1996
- Down's Syndrome: Up‐Regulation of β‐Amyloid Protein Precursor and τ mRNAs and Their Defective CoordinationJournal of Neurochemistry, 1994
- S6 Phosphorylation and the p70s6k/p85s6kCritical Reviews in Biochemistry and Molecular Biology, 1994
- Brain Levels of Microtubule‐Associated Protein τ Are Elevated in Alzheimer's Disease: A Radioimmuno‐Slot‐Blot Assay for Nanograms of the ProteinJournal of Neurochemistry, 1992
- Neuropathological stageing of Alzheimer-related changesActa Neuropathologica, 1991