Interaction of ethanol, prostacyclin, and aspirin in determining human platelet reactivity in vitro.
- 1 July 1988
- journal article
- abstracts
- Published by Ovid Technologies (Wolters Kluwer Health) in Arteriosclerosis: An Official Journal of the American Heart Association, Inc.
- Vol. 8 (4), 436-441
- https://doi.org/10.1161/01.atv.8.4.436
Abstract
Ethanol partitions into cellular membranes and alters membrane-associated phenomena in numerous cell types. Since platelet aggregation and its inhibition by prostacyclin are mediated by membrane-associated receptors and enzymes, we examined the interaction of ethanol, prostacyclin, and aspirin on human platelet reactivity. Using platelet-rich plasma, we examined the effect of increasing concentrations of ethanol (0.05% to 1.0%) on the platelet-inhibitory effects of a submaximal dose (5 x 10(-10) M) of prostacyclin, the concomitant production of cyclic 3',5'-adenosine monophosphate (AMP), and the release of thromboxane A2. Ethanol alone had little effect on platelet aggregation induced by 5 micrograms/ml collagen; however, it potentiated the inhibitory effect of prostacyclin on platelet aggregation in a dose-dependent manner in the range of 0.05% to 1.0% ethanol. Whereas prostacyclin increased platelet cyclic AMP levels, ethanol had no further effect on cyclic AMP levels. Ethanol alone reduced thromboxane A2 generation, but this effect could not totally account for the observed interaction of ethanol and prostacyclin on aggregation, since aspirin did not totally abolish the interaction. The dose range in which the ethanol/prostacyclin/aspirin interactions occur encompasses the plasma levels of ethanol that may be achieved by the consumption of alcoholic beverages. The results may, in part, explain the dose-related physiological and pathological consequences of chronic alcohol consumption on the cardiovascular system.Keywords
This publication has 3 references indexed in Scilit:
- Prostacyclin and arterial wall biology.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1982
- Effects of prostacyclin (PGX) on cyclic AMP concentrations in human plateletsProstaglandins, 1977
- Modulation of human platelet adenylate cyclase by prostacyclin (PGX)Prostaglandins, 1977