Rat aversion to isoflurane versus carbon dioxide
Open Access
- 23 February 2013
- journal article
- Published by The Royal Society in Biology Letters
- Vol. 9 (1), 20121000
- https://doi.org/10.1098/rsbl.2012.1000
Abstract
Some experts suggest that sedation of laboratory rodents with isoflurane before euthanasia with carbon dioxide (CO2) is a humane alternative to euthanasia with CO2alone, but little research has compared aversion with these agents. Albino rats were tested in a light–dark box where they had the choice between remaining in a dark compartment filling with isoflurane or CO2, or escaping to a lit compartment. Experiment 1 validated the procedure by confirming that rats responded to agent and light intensity. In experiment 2, 9/16 and 0/16 rats remained in the dark compartment until recumbent when initially exposed to isoflurane and CO2, respectively. In experiment 3, more rats remained in the dark compartment until recumbent during initial (10/16) versus re-exposure (1/16) to isoflurane. These results indicate that initial exposure to CO2is more aversive than isoflurane, and that re-exposure to isoflurane is more aversive than initial exposure. We conclude that sedation with isoflurane is a refinement over euthanasia with CO2alone for rats that have not been previously exposed to inhalant anaesthetics.Keywords
This publication has 16 references indexed in Scilit:
- Brief light as a practical aversive stimulus for the albino ratBehavioural Brain Research, 2010
- Rat aversion to induction with inhalant anaestheticsApplied Animal Behaviour Science, 2009
- The validity of using an approach-avoidance test to measure the strength of aversion to carbon dioxide in ratsApplied Animal Behaviour Science, 2008
- Effect of flow rate on aversion to gradual-fill carbon dioxide exposure in ratsApplied Animal Behaviour Science, 2008
- Rats avoid exposure to carbon dioxide and argonApplied Animal Behaviour Science, 2007
- Behavioural responses of rats to gradual-fill carbon dioxide euthanasia and reduced oxygen concentrationsApplied Animal Behaviour Science, 2006
- Emergence delirium: a re-emerging interestSeminars in Anesthesia, Perioperative Medicine and Pain, 2006
- Comparison of 60‐Hz electric fields and incandescent light as aversive stimuli controlling the behavior of ratsBioelectromagnetics, 1989
- The sensitivity of albino rats to lights of different wavelength: A behavioral assessmentVision Research, 1972
- Aversion thresholds and aversion difference limens for white light in albino and hooded rats.Journal of Experimental Psychology, 1969