Detection of Salmonella Enteritidis in Equine Feces using the Polymerase Chain Reaction and Genus-Specific Oligonucleotide Primers
- 1 April 1995
- journal article
- Published by SAGE Publications in Journal of Veterinary Diagnostic Investigation
- Vol. 7 (2), 219-222
- https://doi.org/10.1177/104063879500700209
Abstract
Salmonella was identified in feces from horses, using the polymerase chain reaction (PCR) and genus-specific oligonucleotide primers. Feces from healthy horses were determined to be culture negative and PCR negative for Salmonella. Fecal samples were inoculated with known numbers of colony-forming units (CFU) of S. enteritidis. The fecal samples were enriched overnight in tetrathionate broth, and then DNA was extracted and amplified by PCR using genus-specific primers. Sensitivity of the assay extended to 100 CFU Salmonella enteritidis/g feces; sensitivity of microbiologic culture with enrichment extended to 100 CFU Salmonella enteritidis/g feces. Feces that were not inoculated with S. enteritidis were negative by the PCR. Detection of salmonellae in feces was possible using the PCR within 24 hours from the time of submission of samples. Because samples were enriched, isolates were available for determining antibiograms and serologic grouping or typing.Keywords
This publication has 10 references indexed in Scilit:
- Genus-Specific Detection of Salmonellae using the Polymerase Chain Reaction (PCR)Journal of Veterinary Diagnostic Investigation, 1993
- The magnetic immuno polymerase chain reaction assay for direct detection of salmonellae in fecal samplesJournal of Clinical Microbiology, 1992
- Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of SalmonellaMolecular and Cellular Probes, 1992
- Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactionsJournal of Clinical Microbiology, 1990
- Colitis: Differential diagnosis and treatmentEquine Veterinary Journal, 1986
- CASE-CONTROL STUDY OF RISK FACTORS ASSOCIATED WITH ISOLATION OF SALMONELLA SAINTPAUL IN HOSPITALIZED HORSESAmerican Journal of Epidemiology, 1984
- Complete nucleotide sequence and identification of membrane components of the histidine transport operon of S. typhimuriumNature, 1982
- Bovine salmonellosisJournal of Dairy Research, 1977