Suppression of HIV replication using RNA interference against HIV‐1 integrase
- 15 June 2007
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 581 (17), 3253-3259
- https://doi.org/10.1016/j.febslet.2007.06.011
Abstract
RNA interference (RNAi) has become one of the most powerful and popular approach on gene silencing in clinical research study especially in virology due to the gene-specific suppression property of small interfering RNA (siRNA). In this report, we demonstrate that expression of vector-mediated small hairpin RNA (shRNA) against human immunodeficiency virus type 1 (HIV-1) integrase (IN), one of the three important enzymes in HIV infection by controlling the integration of viral RNA to host DNA, could suppress the protein synthesis of EGFP-tagged IN in HeLa cell model efficiently. Furthermore, we show that IN shRNA can successfully reduce the HIV particles production in 293T cells at the level similar to the positive control of HIV-1 tat shRNA. These results provide the therapeutic possibility of HIV replication using RNAi against HIV-1 integrase.Keywords
This publication has 58 references indexed in Scilit:
- Mutation in the loop C-terminal to the cyclophilin A binding site of HIV-1 capsid protein disrupts proper virus assembly and infectivityRetrovirology, 2007
- Silencing of HIV-1 with RNA Interference: a Multiple shRNA ApproachMolecular Therapy, 2006
- Effective Suppression of Human Immunodeficiency Virus Type 1 through a Combination of Short- or Long-Hairpin RNAs Targeting Essential Sequences for Retroviral IntegrationJournal of Virology, 2006
- Structural basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assemblyProceedings of the National Academy of Sciences, 2006
- Gag Regulates Association of Human Immunodeficiency Virus Type 1 Envelope with Detergent-Resistant MembranesJournal of Virology, 2006
- Long-Term Inhibition of HIV-1 Infection in Primary Hematopoietic Cells by Lentiviral Vector Delivery of a Triple Combination of Anti-HIV shRNA, Anti-CCR5 Ribozyme, and a Nucleolar-Localizing TAR DecoyMolecular Therapy, 2005
- Initial Cleavage of the Human Immunodeficiency Virus Type 1 GagPol Precursor by Its Activated Protease Occurs by an Intramolecular MechanismJournal of Virology, 2004
- Requirement for Integrase during Reverse Transcription of Human Immunodeficiency Virus Type 1 and the Effect of Cysteine Mutations of Integrase on Its Interactions with Reverse TranscriptaseJournal of Virology, 2004
- The gene-silencing efficiency of siRNA is strongly dependent on the local structure of mRNA at the targeted regionBiochemical and Biophysical Research Communications, 2004
- Killing the messenger: short RNAs that silence gene expressionNature Reviews Molecular Cell Biology, 2003