Optical Projection Tomography of Vertebrate Embryo Development
- 1 June 2011
- journal article
- Published by Cold Spring Harbor Laboratory in Cold Spring Harbor Protocols
- Vol. 2011 (6), pdb.top116-94
- https://doi.org/10.1101/pdb.top116
Abstract
INTRODUCTION: This article includes a brief introduction to optical projection tomography (OPT) as a method for imaging whole vertebrate embryos. To place OPT in its proper context within the field of three-dimensional (3D) embryo imaging, the article starts with a brief overview of the previously existing techniques, both destructive and nondestructive. Next is a description of how OPT works and its advantages over other approaches. Two practical sections are then presented that consider the applications of OPT and how to get the best results from OPT in terms of specimen preparation and imaging modes.Keywords
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