Rapid diagnosis of experimental meningitis by bacterial heat production in cerebrospinal fluid
Open Access
- 10 October 2007
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Infectious Diseases
- Vol. 7 (1), 116
- https://doi.org/10.1186/1471-2334-7-116
Abstract
Calorimetry is a nonspecific technique which allows direct measurement of heat generated by biological processes in the living cell. We evaluated the potential of calorimetry for rapid detection of bacterial growth in cerebrospinal fluid (CSF) in a rat model of bacterial meningitis. Infant rats were infected on postnatal day 11 by direct intracisternal injection with either Streptococcus pneumoniae, Neisseria meningitidis or Listeria monocytogenes. Control animals were injected with sterile saline or heat-inactivated S. pneumoniae. CSF was obtained at 18 hours after infection for quantitative cultures and heat flow measurement. For calorimetry, 10 μl and 1 μl CSF were inoculated in calorimetry ampoules containing 3 ml trypticase soy broth (TSB). The mean bacterial titer (± SD) in CSF was 1.5 ± 0.6 × 108 for S. pneumoniae, 1.3 ± 0.3 × 106 for N. meningitidis and 3.5 ± 2.2 × 104 for L. monocytogenes. Calorimetric detection time was defined as the time until heat flow signal exceeded 10 μW. Heat signal was detected in 10-μl CSF samples from all infected animals with a mean (± SD) detection time of 1.5 ± 0.2 hours for S. pneumoniae, 3.9 ± 0.7 hours for N. meningitidis and 9.1 ± 0.5 hours for L. monocytogenes. CSF samples from non-infected animals generated no increasing heat flow (S. pneumoniae ranging from 6.7 to 7.5 Joules, followed by L. monocytogenes (5.6 to 6.1 Joules) and N. meningitidis (3.5 to 4.4 Joules). The lowest detectable bacterial titer by calorimetry was 2 cfu for S. pneumoniae, 4 cfu for N. meningitidis and 7 cfu for L. monocytogenes. By means of calorimetry, detection times of S. pneumoniae and N. meningitidis and Listeria monocytogenes using as little as 10 μl CSF were achieved. Calorimetry is a new diagnostic method allowing rapid and accurate diagnosis of bacterial meningitis from a small volume of CSF.Keywords
This publication has 17 references indexed in Scilit:
- In bacterial meningitis cortical brain damage is associated with changes in parenchymal MMP-9/TIMP-1 ratio and increased collagen type IV degradationNeurobiology of Disease, 2006
- Clinical features, complications, and outcome in adults with pneumococcal meningitis: a prospective case seriesThe Lancet Neurology, 2006
- Community-Acquired Bacterial Meningitis in AdultsNew England Journal of Medicine, 2006
- Delays in the administration of antibiotics are associated with mortality from adult acute bacterial meningitisQJM: An International Journal of Medicine, 2005
- Brain‐Derived Neurotrophic Factor Protects against Multiple Forms of Brain Injury in Bacterial MeningitisThe Journal of Infectious Diseases, 2005
- Clinical Features and Prognostic Factors in Adults with Bacterial MeningitisNew England Journal of Medicine, 2004
- Inhibition of matrix metalloproteinases and tumour necrosis factor alpha converting enzyme as adjuvant therapy in pneumococcal meningitisBrain, 2001
- Nitric Oxide Is Protective in Listeric Meningoencephalitis of RatsInfection and Immunity, 2001
- Acute Bacterial Meningitis in Adults: A 12-Year ReviewMedicine, 2000
- Community-Acquired Bacterial Meningitis: Risk Stratification for Adverse Clinical Outcome and Effect of Antibiotic TimingAnnals of Internal Medicine, 1998