Nautilia nitratireducens sp. nov., a thermophilic, anaerobic, chemosynthetic, nitrate-ammonifying bacterium isolated from a deep-sea hydrothermal vent
- 1 May 2010
- journal article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 60 (5), 1182-1186
- https://doi.org/10.1099/ijs.0.013904-0
Abstract
A thermophilic, anaerobic, chemosynthetic bacterium, designated strain MB-1T, was isolated from the walls of an active deep-sea hydrothermal vent chimney on the East Pacific Rise at ° 50′ N 10 ° 17′ W. The cells were Gram-negative-staining rods, approximately 1–1.5 μm long and 0.3–0.5 μm wide. Strain MB-1T grew at 25–65 °C (optimum 55 °C), with 10–35 g NaCl l−1 (optimum 20 g l−1) and at pH 4.5–8.5 (optimum pH 7.0). Generation time under optimal conditions was 45.6 min. Growth occurred under chemolithoautotrophic conditions with H2 as the energy source and CO2 as the carbon source. Nitrate was used as the electron acceptor, with resulting production of ammonium. Thiosulfate, sulfur and selenate were also used as electron acceptors. No growth was observed in the presence of lactate, peptone or tryptone. Chemo-organotrophic growth occurred in the presence of acetate, formate, Casamino acids, sucrose, galactose and yeast extract under a N2/CO2 gas phase. The G+C content of the genomic DNA was 36.0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to Nautilia profundicola AmHT, Nautilia abyssi PH1209T and Nautilia lithotrophica 525T (95, 94 and 93 % sequence identity, respectively). On the basis of phylogenetic, physiological and genetic considerations, it is proposed that the organism represents a novel species within the genus Nautilia, Nautilia nitratireducens sp. nov. The type strain is MB-1T (=DSM 22087T =JCM 15746T).Keywords
This publication has 14 references indexed in Scilit:
- Nautilia abyssi sp. nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal ventInternational Journal of Systematic and Evolutionary Microbiology, 2009
- Nautilia profundicola sp. nov., a thermophilic, sulfur-reducing epsilonproteobacterium from deep-sea hydrothermal ventsInternational Journal of Systematic and Evolutionary Microbiology, 2008
- The versatile ε-proteobacteria: key players in sulphidic habitatsNature Reviews Microbiology, 2006
- Caminibacter mediatlanticus sp. nov., a thermophilic, chemolithoautotrophic, nitrate-ammonifying bacterium isolated from a deep-sea hydrothermal vent on the Mid-Atlantic RidgeInternational Journal of Systematic and Evolutionary Microbiology, 2005
- Lebetimonas acidiphila gen. nov., sp. nov., a novel thermophilic, acidophilic, hydrogen-oxidizing chemolithoautotroph within the ‘Epsilonproteobacteria’, isolated from a deep-sea hydrothermal fumarole in the Mariana ArcInternational Journal of Systematic and Evolutionary Microbiology, 2005
- Thermovibrio ammonificans sp. nov., a thermophilic, chemolithotrophic, nitrate-ammonifying bacterium from deep-sea hydrothermal ventsInternational Journal of Systematic and Evolutionary Microbiology, 2004
- Caminibacter profundus sp. nov., a novel thermophile of Nautiliales ord. nov. within the class ‘Epsilonproteobacteria’, isolated from a deep-sea hydrothermal ventInternational Journal of Systematic and Evolutionary Microbiology, 2004
- SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogenyBioinformatics, 1996
- WWW-query: An on-line retrieval system for biological sequence banksBiochimie, 1996
- A least squares algorithm for fitting additive trees to proximity dataPsychometrika, 1983