Plectin isoform 1b mediates mitochondrion–intermediate filament network linkage and controls organelle shape
Open Access
- 9 June 2008
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 181 (6), 903-911
- https://doi.org/10.1083/jcb.200710151
Abstract
Plectin is a versatile intermediate filament (IF)–bound cytolinker protein with a variety of differentially spliced isoforms accounting for its multiple functions. One particular isoform, plectin 1b (P1b), remains associated with mitochondria after biochemical fractionation of fibroblasts and cells expressing exogenous P1b. Here, we determined that P1b is inserted into the outer mitochondrial membrane with the exon 1b–encoded N-terminal sequence serving as a mitochondrial targeting and anchoring signal. To study P1b-related mitochondrial functions, we generated mice that selectively lack this isoform but express all others. In primary fibroblasts and myoblasts derived from these mice, we observe a substantial elongation of mitochondrial networks, whereas other mitochondrial properties remain largely unaffected. Normal morphology of mitochondria could be restored by isoform-specific overexpression of P1b in P1b-deficient as well as plectin-null cells. We propose a model where P1b both forms a mitochondrial signaling platform and affects organelle shape and network formation by tethering mitochondria to IFs.Keywords
This publication has 27 references indexed in Scilit:
- Plectin-controlled keratin cytoarchitecture affects MAP kinases involved in cellular stress response and migrationThe Journal of cell biology, 2006
- Plectin scaffolds recruit energy-controlling AMP-activated protein kinase (AMPK) in differentiated myofibresJournal of Cell Science, 2006
- Mutations in the neurofilament light gene linked to Charcot‐Marie‐Tooth disease cause defects in transportJournal of Neurochemistry, 2005
- Plectin-RACK1 (Receptor for Activated C Kinase 1) ScaffoldingJournal of Biological Chemistry, 2004
- Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosisThe Journal of cell biology, 2004
- Signal-Anchor Domains of Proteins of the Outer Membrane of MitochondriaPublished by Elsevier BV ,2003
- Predicting transmembrane protein topology with a hidden markov model: application to complete genomesJournal of Molecular Biology, 2001
- Plectin Transcript Diversity: Identification and Tissue Distribution of Variants with Distinct First Coding Exons and Rodless IsoformsGenomics, 1997
- Import and Insertion of Proteins into the Mitochondrial Outer MembraneJBIC Journal of Biological Inorganic Chemistry, 1995
- Primary mouse myoblast purification, characterization, and transplantation for cell-mediated gene therapy.The Journal of cell biology, 1994