Genetic Analysis of an Ambler Class A Extended-Spectrum Beta-Lactamase from Capnocytophaga ochracea
Open Access
- 1 February 2004
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (2), 888-890
- https://doi.org/10.1128/jcm.42.2.888-890.2004
Abstract
A beta-lactamase gene ( cfxA3 , 966 bp) was isolated from a beta-lactam-resistant Capnocytophaga ochracea clinical isolate and amplified using primers from the cfxA gene of Bacteroides vulgatus . The MICs of third-generation cephalosporins were much higher than those of the transconjugant Escherichia coli strain. The deduced protein sequence, by comparison with CfxA2 of Prevotella intermedia , had a Y239D substitution and possessed the characteristics of a class A, group 2e beta-lactamase.Keywords
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