Sapinmusaponins F−J, Bioactive Tirucallane-Type Saponins from the Galls ofSapindusmukorossi

Abstract

Five new tirucallane-type saponins, sapinmusaponins F−J (1−5), were isolated from the galls of Sapindus mukorossi. The structures of these saponins were elucidated on the basis of spectroscopic analysis including 1D and 2D NMR techniques (¹H−¹H COSY, HMQC, HMBC, TOCSY, and NOESY). Compounds 1−5 showed anti-platelet-aggregation effects, but no obvious cytotoxic activity for platelets as assayed by lactate dehydrogenase (LDH) leakage. Compounds 1−5 also showed moderate activity in a 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced Epstein−Barr virus early antigen (EBV-EA) activation assay.