CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells
Top Cited Papers
- 6 January 2017
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 355 (6320)
- https://doi.org/10.1126/science.aah7111
Abstract
The human genome produces thousands of long noncoding RNAs (lncRNAs)—transcripts >200 nucleotides long that do not encode proteins. Although critical roles in normal biology and disease have been revealed for a subset of lncRNAs, the function of the vast majority remains untested. We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth-modifying function exclusively in one cell type. We further found that lncRNA knockdown can perturb complex transcriptional networks in a cell type–specific manner. These data underscore the functional importance and cell type specificity of many lncRNAs.This publication has 70 references indexed in Scilit:
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