Production of Deuterated Lutein by Chlorella protothecoides and its Detection by Mass Spectrometric Methods
- 6 July 2006
- journal article
- Published by Springer Science and Business Media LLC in Biotechnology Letters
- Vol. 28 (17), 1371-1375
- https://doi.org/10.1007/s10529-006-9105-8
Abstract
Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed 58% replacement of hydrogen by deuterium atoms as indicated by the molecular mass cluster at around m/z 599. The rapidly growing microalga had much higher levels (58%) of deuterium substitution relative to previously reported (9–15%) natural sources of lutein.Keywords
This publication has 8 references indexed in Scilit:
- Production of Deuterated Zeaxanthin by Flavobacterium Multivorum and its Detection by Resonance Raman and Mass Spectrometric MethodsBiotechnology Letters, 2005
- Hyphenation of capillary high-performance liquid chromatography to microcoil magnetic resonance spectroscopy—determination of various carotenoids in a small-sized spinach sampleJournal of Pharmaceutical and Biomedical Analysis, 2005
- Macular pigment optical density and its relationship with serum and dietary levels of lutein and zeaxanthinArchives of Biochemistry and Biophysics, 2004
- Accumulation of astaxanthin and lutein in Chlorella zofingiensis (Chlorophyta)Applied Microbiology and Biotechnology, 2003
- A mutant of the green alga Dunaliella salina constitutively accumulates zeaxanthin under all growth conditionsBiotechnology & Bioengineering, 2002
- High‐Yield Production of Lutein by the Green Microalga Chlorella protothecoidesin Heterotrophic Fed‐Batch CultureBiotechnology Progress, 2002
- Identification and Quantitation of Carotenoids and their Metabolites in the Tissues of the Human EyeExperimental Eye Research, 2001
- Preliminary identification of the human macular pigmentVision Research, 1985