Factors affecting variations in the detailed fatty acid profile of Mediterranean buffalo milk determined by 2-dimensional gas chromatography
- 1 April 2017
- journal article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 100 (4), 2564-2576
- https://doi.org/10.3168/jds.2016-11696
Abstract
Buffalo milk is the world's second most widely produced milk, and increasing attention is being paid to its composition, particularly the fatty acid profile. The objectives of the present study were (1) to characterize the fatty acid composition of Mediterranean buffalo milk, and (2) to investigate potential sources of variation in the buffalo milk fatty acid profile. We determined the profile of 69 fatty acid traits in 272 individual samples of Mediterranean buffalo milk using gas chromatography. In total, 51 individual fatty acids were identified: 24 saturated fatty acids, 13 monounsaturated fatty acids, and 14 polyunsaturated fatty acids. The major individual fatty acids in buffalo milk were in the order 16:0, 18:1 cis-9, 14:0, and 18:0. Saturated fatty acids were the predominant fraction in buffalo milk fat (70.49%); monounsaturated and polyunsaturated fatty acids were at 25.95 and 3.54%, respectively. Adopting a classification based on carbon-chain length, we found that medium-chain fatty acids (11-16 carbons) represented the greater part (53.7%) of the fatty acid fraction of buffalo milk, whereas long-chain fatty acids (17-24 carbons) and short-chain fatty acids (4-10 carbons) accounted for 32.73 and 9.72%, respectively. The n-3 and n-6 fatty acids were 0.46 and 1.77%, respectively. The main conjugated linoleic acid, rumenic acid, represented 0.45% of total milk fatty acids. Herd/test date and stage of lactation were confirmed as important sources of variation in the fatty acid profile of buffalo milk. The percentages of short-chain and medium-chain fatty acids in buffalo milk increased in early lactation (+0.6 and +3.5%, respectively), whereas long-chain fatty acids decreased (-4.2%). The only exception to this pattern was butyric acid, which linearly decreased from the beginning of lactation, confirmation that its synthesis is independent of malonyl-CoA. These results seem to suggest that in early lactation the mobilization of energy reserves may have less influence on the fatty acid profile of buffalo milk than that of cow milk, probably due to a shorter and less severe period of negative energy balance. Parity affected the profiles of a few traits and had the most significant effects on branched-chain fatty acids. This work provided a detailed overview of the fatty acid profile in buffalo milk including also those fatty acids present in small concentrations, which may have beneficial effects for human health. Our results contributed also to increase the knowledge about the effects of some of the major factors affecting buffalo production traits and fatty acid concentrations in milk, and consequently its technological and nutritional properties.Keywords
This publication has 38 references indexed in Scilit:
- Investigating mutual relationship among milk fatty acids by multivariate factor analysis in dairy cowsLivestock Science, 2016
- Intake of saturated and trans unsaturated fatty acids and risk of all cause mortality, cardiovascular disease, and type 2 diabetes: systematic review and meta-analysis of observational studiesBMJ, 2015
- Characteristics of dairy farms in the North-Eastern part of Italy: rations, milk yield and nutrients excretionItalian Journal of Animal Science, 2009
- Effect of Lactation Stage on the Odd- and Branched-Chain Milk Fatty Acids of Dairy Cattle Under Grazing and Indoor ConditionsJournal of Dairy Science, 2008
- Results of stereospecific analysis of triacylglycerol fraction from donkey, cow, ewe, goat and buffalo milkJournal of Food Composition and Analysis, 2008
- Genetic Evaluations of Dairy Bulls for Daughter Energy Balance Profiles Using Linear Type Scores and Body Condition Score Analyzed Using Random RegressionJournal of Dairy Science, 2003
- Characterization and biological activity of gangliosides in buffalo milkBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2002
- Effects of Age and Calving Season on Lactation Curves of Milk Production Traits in Italian Water BuffaloesJournal of Dairy Science, 2002
- Phospholipids in milk and dairy productsInternational Journal of Dairy Technology, 1987
- Influence of stage of lactation on the triacylglycerol composition of buffalo milk fatLipids, 1981