Age-related changes in platelet function are more profound in women than in men
Open Access
- 15 July 2015
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 5 (1), 12235
- https://doi.org/10.1038/srep12235
Abstract
Age is a risk factor for cardiovascular disease (CVD), however the effect of age on platelet function remains unclear. Ideally, platelet function should be assayed under flow and shear conditions that occur in vivo. Our study aimed to characterise the effect of age on platelet translocation behaviour using a novel flow-based assay that measures platelet function in less than 200 μl of blood under conditions of arterial shear. Blood from males (n = 53) and females (n = 56), ranging in age from 19–82 and 21–70 respectively were perfused through custom-made parallel plate flow chambers coated with immobilised human von Willebrand Factor (VWF) under arterial shear (1,500s−1). Platelet translocation behaviour on VWF was recorded by digital-image microscopy and analysed. The study showed that aging resulted in a significant decrease in the number of platelet tracks, translocating platelets and unstable platelet interactions with VWF. These age related changes in platelet function were more profound in women than in men indicating that age and gender significantly impacts on platelet interactions with VWF.This publication has 23 references indexed in Scilit:
- Microfluidic device to study arterial shear-mediated platelet-surface interactions in whole blood: reduced sample volumes and well-characterised protein surfacesBiomedical Microdevices, 2010
- Loss of estrogen receptor β decreases mitochondrial energetic potential and increases thrombogenicity of platelets in aged female miceAGE, 2009
- Reconfirmation of Preanalytical Variables and Establishment of Reference Intervals of Platelet Function Analyzer-100 Closure Times in Korean AdultsAnnals of Laboratory Medicine, 2007
- Adhesion Mechanisms in Platelet FunctionCirculation Research, 2007
- Hemostasis and agingCritical Reviews in Oncology/Hematology, 2006
- Altered Platelet Function Detected by Flow CytometryArteriosclerosis, Thrombosis, and Vascular Biology, 1997
- Platelet Adhesion to Multimeric and Dimeric von Willebrand Factor and to Collagen Type III Preincubated With von Willebrand FactorArteriosclerosis, Thrombosis, and Vascular Biology, 1996
- The Effect of Ageing on Platelet Function and Fibrinolytic ActivityAngiology, 1995
- Whole-Blood Platelet Aggregation Predicts In Vitro and In Vivo Primary Hemostatic Function in the ElderlyArteriosclerosis, Thrombosis, and Vascular Biology, 1995
- The Estrogen Receptor Is Present in Human MegakaryocytesAnnals of the New York Academy of Sciences, 1994