Potential of polymerase chain reaction and galactomannan for the diagnosis of invasive aspergillosis in patients with febrile neutropenia
- 7 April 2015
- Vol. 58 (6), 343-349
- https://doi.org/10.1111/myc.12322
Abstract
The incidence of invasive aspergillosis (IA) has increased over the last years, especially in immuncompromised patients with high mortality rates. Because of difficulties about the diagnosis; serological methods [galactomannan (GM) antigen test] and polymerase chain reaction (PCR) developed in recent years. MycAssay Aspergillus PCR performance in the diagnosis of IA was evaluated and compared with the GM and in-house PCR. This study was conducted with 358 serum samples obtained from 99 patient with febrile neutropenic episodes who were followed in haematology and bone marrow transplantation units. Patients were classified by the European Organization for the Research and Treatment of Cancer/Mycoses Study Group criteria, 18 of them is proven and probable IA. GM antigen test and two different real-time PCR; one of them is fist commercial PCR for IA; Mycassay Aspergillus and the other one is in-house real-time PCR performed. Sensitivity values were Mycassay Aspergillus PCR, in-house PCR, and GM 65.38%, 11.53% and 23.07%, respectively. The high sensitivity obtained from Mycassay Aspergillus PCR and sensitivity is increased by using a combination of diagnostic methods. GM antigen test and real-time PCR could be beneficial for early diagnosis and treatment of IA. For routine usage of PCR as diagnostic assay more studies needed in future.Keywords
Funding Information
- Eskişehir Osmangazi University (2011/11028)
This publication has 25 references indexed in Scilit:
- Hema e-Chart Registry of invasive fungal infections in haematological patients: improved outcome in recent years in mould infectionsClinical Microbiology & Infection, 2013
- Fungal diagnosis: how do we do it and can we do better?Current Medical Research and Opinion, 2013
- Invasive Mycoses: Diagnostic ChallengesAmerican Journal Of Medicine, 2012
- Diagnosis of Invasive Aspergillosis by a Commercial Real-Time PCR Assay for Aspergillus DNA in Bronchoalveolar Lavage Fluid Samples from High-Risk Patients Compared to a Galactomannan Enzyme ImmunoassayJournal of Clinical Microbiology, 2011
- Evaluation of Analytical and Preliminary Clinical Performance of Myconostica MycAssay Aspergillus When Testing Serum Specimens for Diagnosis of Invasive AspergillosisJournal of Clinical Microbiology, 2011
- Risk assessment and prognostic factors for mould-related diseases in immunocompromised patientsJournal of Antimicrobial Chemotherapy, 2010
- Revised Definitions of Invasive Fungal Disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus GroupClinical Infectious Diseases, 2008
- Antifungal Therapy Decreases Sensitivity of theAspergillus Galactomannan Enzyme ImmunoassayClinical Infectious Diseases, 2005
- Prospective Comparison of the Diagnostic Potential of Real-Time PCR, Double-Sandwich Enzyme-Linked Immunosorbent Assay for Galactomannan, and a (1→3)-β- d -Glucan Test in Weekly Screening for Invasive Aspergillosis in Patients with Hematological DisordersJournal of Clinical Microbiology, 2004
- Statistical Methods in Medical ResearchPublished by Wiley ,2002