Generation of Epstein‐Barr Virus (EBV)–Immortalized B Cell Lines
Open Access
- 1 February 2007
- journal article
- unit
- Published by Wiley in Current Protocols in Immunology
- Vol. 76 (1), 7.22.1-7.22.4
- https://doi.org/10.1002/0471142735.im0722s76
Abstract
Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.Keywords
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