Effect of insulin and growth hormone on rat heart and liver oxidative stress in control and caloric restricted animals
- 1 January 2005
- journal article
- research article
- Published by Springer Science and Business Media LLC in Biogerontology
- Vol. 6 (1), 15-26
- https://doi.org/10.1007/s10522-004-7380-0
Abstract
In order to know if insulin-like signalling is involved in the control of oxidative stress in mammalian tissues in relation to aging, ad libitum-fed and caloric restricted Wistar rats were treated during 2 weeks with GH and insulin. The most consistent effect of the hormonal treatments was an increase in plasma IGF-1 levels. Caloric restriction during 6 weeks decreased ROS generation and oxidative DNA damage in heart mitochondria and this was reversed by insulin treatment. The decrease in oxidative damage to liver nuclear DNA induced by caloric restriction was also reversed by GH and insulin. In the liver, however, insulin and GH decreased mitochondrial ROS generation while they increased oxidative damage to mitochondrial DNA. GH and insulin decreased three different markers of oxidative modification of liver proteins, while they increased lipoxidation-dependent markers. This last result is related to the increase in phospholipid unsaturation induced in the liver by both hormones. The results suggest that the idea that insulin-like signalling controls oxidative stress in mammals cannot be generalized since both prooxidant and protective effects of GH and insulin are observed depending on the particular parameter and tissue selected.Keywords
This publication has 36 references indexed in Scilit:
- Growth hormone prevents neuronal loss in the aged rat hippocampusNeurobiology of Aging, 2005
- Mammalian 8-Oxoguanine DNA Glycosylase 1 Incises 8-Oxoadenine Opposite Cytosine in Nuclei and Mitochondria, while a Different Glycosylase Incises 8-Oxoadenine Opposite Guanine in NucleiJournal of Biological Chemistry, 2003
- Extended Longevity in Mice Lacking the Insulin Receptor in Adipose TissueScience, 2003
- IGF-1 receptor regulates lifespan and resistance to oxidative stress in miceNature, 2002
- Lifespan extension by caloric restriction: An aspect of energy metabolismMicroscopy Research and Technique, 2002
- Extension of Life-Span by Loss of CHICO, a Drosophila Insulin Receptor Substrate ProteinScience, 2001
- The effects of growth hormone and IGF-1 deficiency on cerebrovascular and brain ageingJournal of Anatomy, 2000
- Dwarf mice and the ageing processNature, 1996
- The Advanced Glycation End Product, N∊-(Carboxymethyl)lysine, Is a Product of both Lipid Peroxidation and Glycoxidation ReactionsJournal of Biological Chemistry, 1996
- Clonidine Potentiates the Growth Hormone (GH) Response to GH-Releasing Hormone in Norepinephrine Synthesis-Inhibited Rats: Evidence for an Alpha-2-Adrenergic Control of Hypothalamic Release of SomatostatinNeuroendocrinology, 1993