Characterisation of a plant 3‐phosphoinositide‐dependent protein kinase‐1 homologue which contains a pleckstrin homology domain
Open Access
- 28 May 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 451 (3), 220-226
- https://doi.org/10.1016/s0014-5793(99)00556-6
Abstract
A plant homologue of mammalian 3‐phosphoinositide‐dependent protein kinase‐1 (PDK1) has been identified in Arabidopsis and rice which displays 40% overall identity with human 3‐phosphoinositide‐dependent protein kinase‐1. Like the mammalian 3‐phosphoinositide‐dependent protein kinase‐1, Arabidopsis 3‐phosphoinositide‐dependent protein kinase‐1 and rice 3‐phosphoinositide‐dependent protein kinase‐1 possess a kinase domain at N‐termini and a pleckstrin homology domain at their C‐termini. Arabidopsis 3‐phosphoinositide‐dependent protein kinase‐1 can rescue lethality in Saccharomyces cerevisiae caused by disruption of the genes encoding yeast 3‐phosphoinositide‐dependent protein kinase‐1 homologues. Arabidopsis 3‐phosphoinositide‐dependent protein kinase‐1 interacts via its pleckstrin homology domain with phosphatidic acid, PtdIns3P, PtdIns(3,4,5)P3 and PtdIns(3,4)P2 and to a lesser extent with PtdIns(4,5)P2 and PtdIns4P. Arabidopsis 3‐phosphoinositide‐dependent protein kinase‐1 is able to activate human protein kinase Bα (PKB/AKT) in the presence of PtdIns(3,4,5)P3. Arabidopsis 3‐phosphoinositide‐dependent protein kinase‐1 is only the second plant protein reported to possess a pleckstrin homology domain and the first plant protein shown to bind 3‐phosphoinositides.Keywords
This publication has 24 references indexed in Scilit:
- Intracellular signalling: PDK1 – a kinase at the hub of thingsCurrent Biology, 1999
- Detailed analysis of the turnover of polyphosphoinositides and phosphatidic acid upon activation of phospholipases C and D in Chlamydomonas cells treated with non-permeabilizing concentrations of mastoparanPlanta, 1998
- Identification and analysis of PH domain-containing targets of phosphatidylinositol 3-kinase using a novel in vivo assay in yeastThe EMBO Journal, 1998
- Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase BαCurrent Biology, 1997
- Two genes that encode ribosomal‐protein S6 kinase homologs are induced by cold or salinity stress in Arabidopsis thalianaFEBS Letters, 1995
- Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-JunNature, 1994
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Improved method for high efficiency transformation of intact yeast cellsNucleic Acids Research, 1992
- The Effect of Phytohormones on Phosphorylation of Ribosomal Proteins in Detached Pumpkin CotyledonsBiochemie und Physiologie der Pflanzen, 1987
- A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistanceMolecular Genetics and Genomics, 1984