In Situ Simultaneous Analysis of Polyacetylenes, Carotenoids and Polysaccharides in Carrot Roots
- 28 July 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 53 (17), 6565-6571
- https://doi.org/10.1021/jf0510440
Abstract
This paper presents an approach to simultaneously analyze polyacetylenes, carotenoids, and polysaccharides in carrot (Daucus carota L.) roots by means of Raman spectroscopy. The components were measured in situ in the plant tissue without any preliminary sample preparation. The analysis is based on the intensive and characteristic key bands observed in the Raman spectrum of carrot root. The molecular structures of the main carrot polyacetylenes, falcarinol and falcarindiol, are similar, but their Raman spectra exhibit specific differences demonstrated by the shift of their −C⋮C− mode from 2258 to 2252 cm-1, respectively. Carotenoids can be identified by −CC− stretching vibrations (about 1520 and 1155 cm-1) of the conjugated system of their polyene chain, whereas the characteristic Raman band at 478 cm-1 indicates the skeletal vibration mode of starch molecule. The other polysaccharide, pectin, can be identified by the characteristic band at 854 cm-1, which is due to the −C−O−C− skeletal mode of α-anomer carbohydrates. The Raman mapping technique applied here has revealed detailed information regarding the relative distribution of polyacetylenes, carotenoids, starch, and pectin in the investigated plant tissues. The distribution of these components varies among various carrot cultivars, and especially a significant difference can be seen between cultivated carrot and the wild relative D. carota ssp. maritimus. Keywords: Daucus carota; NIR-FT-Raman; mapping; falcarinol; falcarindiol; starch; pectin; nondestructive analysis; caroteneKeywords
This publication has 21 references indexed in Scilit:
- Spatial tissue distribution of polyacetylenes in carrot rootThe Analyst, 2005
- Polyacetylenes from the Apiaceae Vegetables Carrot, Celery, Fennel, Parsley, and Parsnip and Their Cytotoxic ActivitiesJournal of Agricultural and Food Chemistry, 2005
- Potential of NIR‐FT‐Raman spectroscopy in natural carotenoid analysisPeptide Science, 2005
- Effects of Genotype, Root Size, Storage, and Processing on Bioactive Compounds in Organically Grown Carrots (Daucus carota L.)Journal of Food Science, 2004
- Health promoting compounds in vegetables and fruits: A systematic approach for identifying plant components with impact on human healthTrends in Food Science & Technology, 2004
- Effects of Boiling and Storage on Dietary Fibre and Digestible Carbohydrates in Various Cultivars of CarrotsJournal of the Science of Food and Agriculture, 1997
- The Influence of Polyacetylenes on the Susceptibility of Carrots to Storage DiseasesJournal of Phytopathology, 1996
- Carotenes in typical and dark orange carrotsJournal of Agricultural and Food Chemistry, 1987
- Location of the antifungal compound falcarindiol in carrot root tissueTransactions of the British Mycological Society, 1979
- Cis-heptadeca-1,9-diene-4,6-diyne-3,8-diol, an antifungal polyacetylene from carrot root tissuePhysiological Plant Pathology, 1978