Quantitative determination of the major metabolite of prostaglandins F1α and F2α in human urine by stable isotope dilution and combined gas chromatography-mass spectrometry
- 31 August 1976
- journal article
- Published by Elsevier BV in Biochemical Medicine
- Vol. 16 (1), 77-94
- https://doi.org/10.1016/0006-2944(76)90011-9
Abstract
An improved gc-ms method has been developed for the measurement of 5α,7α- dihydroxy - 11 - ketotetranorprosta - 1,16 - dioic acid (PGF-M), the major urinary metabolite of PGF1α and PGF2α in man. The assay is based on gas chromatography and selected ion monitoring mass spectrometry, employing a specifically deuterated analog of the metabolite, labeled in the cyclopentane ring, as internal standard.Keywords
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