Pulsed ultrasound associated with gold nanoparticle gel reduces oxidative stress parameters and expression of pro-inflammatory molecules in an animal model of muscle injury
Open Access
- 12 March 2012
- journal article
- Published by Springer Science and Business Media LLC in Journal of Nanobiotechnology
- Vol. 10 (1), 11
- https://doi.org/10.1186/1477-3155-10-11
Abstract
Background Nanogold has been investigated in a wide variety of biomedical applications because of the anti-inflammatory properties. The purpose of this study was to evaluate the effects of TPU (Therapeutic Pulsed Ultrasound) with gold nanoparticles (GNP) on oxidative stress parameters and the expression of pro-inflammatory molecules after traumatic muscle injury. Materials and methods Animals were divided in nine groups: sham (uninjured muscle); muscle injury without treatment; muscle injury + DMSO; muscle injury + GNP; muscle injury + DMSO + GNP; muscle injury + TPU; muscle injury + TPU + DMSO; muscle injury + TPU + GNP; muscle injury + TPU + DMSO + GNP. The ROS production was determined by concentration of superoxide anion, modulation of antioxidant defenses was determined by the activity of superoxide dismutase, catalase and glutathione peroxidase enzymes, oxidative damage determined by formation of thiobarbituric acid-reactive substance and protein carbonyls. The levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as inflammatory parameters. Results Compared to muscle injury without treatment group, the muscle injury + TPU + DMSO + GNP gel group promoted a significant decrease in superoxide anion production and lipid peroxidation levels (p < 0.050). It also showed a significant decrease in TNF-α and IL-1β levels (p < 0.050) when compared to muscle injury without treatment group. Conclusions Our results suggest that TPU + DMSO + GNP gel presents beneficial effects on the muscular healing process, inducing a reduction in the production of ROS and also the expression of pro-inflammatory molecules.This publication has 52 references indexed in Scilit:
- CHARACTERIZATION OF GLUCOSE‐INDUCED GLUCOSE OXIDASE GENE AND PROTEIN EXPRESSION IN Helicoverpa armigera LARVAEArchives of Insect Biochemistry and Physiology, 2012
- Stanozolol treatment decreases the mitochondrial ROS generation and oxidative stress induced by acute exercise in rat skeletal muscleJournal of Applied Physiology, 2011
- The role of Ca2+ in ultrasound-elicited bioeffects: progress, perspectives and prospectsDrug Discovery Today, 2010
- Interleukin-6 Is an Essential Regulator of Satellite Cell-Mediated Skeletal Muscle HypertrophyCell Metabolism, 2008
- Ultrasound–biophysics mechanismsProgress in Biophysics and Molecular Biology, 2007
- Inflammatory processes in muscle injury and repairAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2005
- Pertussis toxin‐sensitive Gαi protein and ERK‐dependent pathways mediate ultrasound promotion of osteogenic transcription in human osteoblasts1FEBS Letters, 2003
- Gold compound auranofin inhibits IκB kinase (IKK) by modifying Cys-179 of IKKβ subunitExperimental & Molecular Medicine, 2003
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970