Concatenated logic gates using four coupled biocatalysts operating in series
Open Access
- 14 November 2006
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 103 (46), 17160-17163
- https://doi.org/10.1073/pnas.0608319103
Abstract
The assembly of three concatenated enzyme-based logic gates consisting of OR, AND, XOR is described. Four biocatalysts, acetylcholine esterase, choline oxidase, microperoxidase-11, and the NAD+-dependent glucose dehydrogenase, are used to assemble the gates. Four inputs that include acetylcholine, butyrylcholine, O2, and glucose are used to drive the concatenated-gates system. The cofactor NAD+, and its reduced 1,4-dihydro form, NADH, are used as a reporter couple, and these provide an optical output for the gates. The modulus of the absorbance changes of NADH is used as a readout signal.Keywords
This publication has 55 references indexed in Scilit:
- PlantTFDB: a comprehensive plant transcription factor databaseNucleic Acids Research, 2007
- Distinct Biphasic mRNA Changes in Response to Asian Soybean Rust InfectionMolecular Plant-Microbe Interactions®, 2007
- Local coexpression domains in the genome of rice show no microsynteny with Arabidopsis domainsPlant Molecular Biology, 2007
- Genome-wide patterns of single-feature polymorphism in Arabidopsis thalianaProceedings of the National Academy of Sciences of the United States of America, 2007
- A Novel Nucleus-Encoded Chloroplast Protein, PIFI, Is Involved in NAD(P)H Dehydrogenase Complex-Mediated Chlororespiratory Electron Transport in ArabidopsisPlant Physiology, 2007
- Phosphorus Stress in Common Bean: Root Transcript and Metabolic ResponsesPlant Physiology, 2007
- MEME: discovering and analyzing DNA and protein sequence motifsNucleic Acids Research, 2006
- High-density haplotyping with microarray-based expression and single feature polymorphism markers in ArabidopsisGenome Research, 2006
- An autonomous molecular computer for logical control of gene expressionNature, 2004
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997