A Monoclonal Antibody to Cellular Transglutaminase
- 1 January 1985
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 4 (2), 179-186
- https://doi.org/10.1089/hyb.1985.4.179
Abstract
A cellular enzyme-linked immunosorbent assay was developed for estimating cellular transglutaminase in situ using a monoclonal antibody produced to tissue transglutaminase. The minimum level of detection of TGase was 2-5 ng. The enzyme was present in greater amounts in WI-38 and IMR90 cells than in their simian virus-transformed counterparts. The levels of TGase in the virus-transformed cells increased significantly when the cells were grown in the presence of sodium butyrate to induce enzyme activity. Staining of confluent WI-38 cells by indirect immunofluorescence using the monoclonal antibody showed microscopic fibers suggesting that the enzyme may be associated with detergent-insoluble componentsThis publication has 8 references indexed in Scilit:
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