WEE2 Is an Oocyte-Specific Meiosis Inhibitor in Rhesus Macaque Monkeys1
Open Access
- 1 June 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 82 (6), 1190-1197
- https://doi.org/10.1095/biolreprod.109.081984
Abstract
WEE1 homolog 2 (WEE2, also known as WEE1B) is a newly identified member of the WEE kinase family that is conserved from yeast to humans. The aim of the present study was to determine the spatiotemporal expression pattern and the function of WEE2 during oocyte maturation in a nonhuman primate species, the rhesus macaque. Among 11 macaque tissues examined, WEE2 transcript is predominantly expressed in the ovary and only weakly detectable in the testis. Within the ovary, WEE2 mRNA is exclusively localized in the oocyte and appears to accumulate during folliculogenesis, reaching the highest level in preovulatory follicles. Microinjection of a full-length WEE2-GFP (green fluorescent protein) fusion mRNA indicates a specific nuclear localization of WEE2 protein in both growing and fully grown germinal vesicle (GV)-intact oocytes. Taking the long double-stranded RNA-mediated RNA interference approach, we found that down-regulation of WEE2 led to meiotic resumption in a subset of GV oocytes even in the presence of a phosphodiesterase 3 inhibitor. On the other hand, overexpression of WEE2 delays the reentry of oocytes into meiosis in both mice and monkeys. These findings suggest that WEE2 is a conserved oocyte-specific meiosis inhibitor that functions downstream of cAMP in nonhuman primates.Keywords
This publication has 44 references indexed in Scilit:
- Wee1B, Myt1, and Cdc25 function in distinct compartments of the mouse oocyte to control meiotic resumptionThe Journal of cell biology, 2010
- Generation of mouse oocytes defective in cAMP synthesis and degradation: Endogenous cyclic AMP is essential for meiotic arrestDevelopmental Biology, 2008
- Mechanism for inactivation of the mitotic inhibitory kinase Wee1 at M phaseProceedings of the National Academy of Sciences of the United States of America, 2007
- An active protein kinase A (PKA) is involved in meiotic arrest of rat growing oocytesReproduction, 2006
- New Pathways from PKA to the Cdc2/cyclin B Complex in Oocytes: Wee1B as a Potential PKA SubstrateCell Cycle, 2005
- Absence of non-specific effects of RNA interference triggered by long double-stranded RNA in mouse oocytesDevelopmental Biology, 2005
- Cellular, biochemical and molecular mechanisms regulating oocyte maturationMolecular and Cellular Endocrinology, 2005
- Long dsRNA and silent genes strike back:RNAi in mouse oocytes and early embryosCytogenetic and Genome Research, 2004
- Cdc25b phosphatase is required for resumption of meiosis during oocyte maturationNature Genetics, 2002
- Protein phosphorylation/dephosphorylation in the meiotic cell cycle of mammalian oocytesReviews of Reproduction, 1996