Nanoscale Resolution in the Focal Plane of an Optical Microscope
- 15 April 2005
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 94 (14), 143903
- https://doi.org/10.1103/physrevlett.94.143903
Abstract
Utilizing single fluorescent molecules as probes, we prove the ability of a far-field microscope to attain spatial resolution down to 16 nm in the focal plane, corresponding to about of the employed wavelength. The optical bandwidth expansion by nearly an order of magnitude is realized by a saturated depletion through stimulated emission of the molecular fluorescent state. We demonstrate that en route to the molecular scale, the resolving power increases with the square root of the saturation level, which constitutes a new law regarding the resolution of an emerging class of far-field light microscopes that are not limited by diffraction.
Keywords
This publication has 12 references indexed in Scilit:
- Strategy for far-field optical imaging and writing without diffraction limitPhysics Letters A, 2004
- Sharper Focus for a Radially Polarized Light BeamPhysical Review Letters, 2003
- High-Resolution Near-Field Raman Microscopy of Single-Walled Carbon NanotubesPhysical Review Letters, 2003
- High Resolution 3D X-Ray Diffraction MicroscopyPhysical Review Letters, 2002
- Focal Spots of SizeOpen Up Far-Field Florescence Microscopy at 33 nm Axial ResolutionPhysical Review Letters, 2002
- Breaking Abbe’s diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapesPhysical Review E, 2001
- Quantum Interferometric Optical Lithography: Exploiting Entanglement to Beat the Diffraction LimitPhysical Review Letters, 2000
- Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopyOptics Letters, 1994
- Super-gain antennas and optical resolving powerIl Nuovo Cimento (1869-1876), 1952
- Beiträge zur Theorie des Mikroskops und der mikroskopischen WahrnehmungArchiv für Mikroskopische Anatomie, 1873