Development and validation of a clinical HPLC method for the quantification of hydroxychloroquine and its metabolites in whole blood
Open Access
- 1 November 2015
- journal article
- Published by Future Science Ltd in Future Science OA
- Vol. 1 (3), FSO26
- https://doi.org/10.4155/fso.15.24
Abstract
Background: Therapeutic drug monitoring for hydroxychloroquine (HCQ) has been suggested to assess nonadherence and optimize treatment efficacy in systemic lupus erythematosus patients. Materials & methods: After protein precipitation, HCQ and its metabolites, desethylhydroxychloroquine and desethylchloroquine were separated on a phenyl column and monitored by fluorescence detection. The method was linear from 50 to 4000 ng/ml for HCQ. The intra-day and inter-day precision of HCQ, desethylhydroxychloroquine and desethylchloroquine ranged from 4.3 to 10.3%. LLOQ was 50 ng/ml for HCQ. Conclusion: The method is very practical and was applied to routinely monitor the steady state whole blood exposure of HCQ and its metabolites in systemic lupus erythematosus patients. It well correlated with our LC–MS/MS and another HPLC method.Keywords
This publication has 23 references indexed in Scilit:
- The Early Protective Effect of Hydroxychloroquine on the Risk of Cumulative Damage in Patients with Systemic Lupus ErythematosusThe Journal of Rheumatology, 2013
- Pharmacogenetics: implications for therapy in rheumatic diseasesNature Reviews Rheumatology, 2011
- Antimalarial treatment may have a time‐dependent effect on lupus survival: Data from a multinational Latin American inception cohortArthritis & Rheumatism, 2010
- Effect of hydroxychloroquine on the survival of patients with systemic lupus erythematosus: data from LUMINA, a multiethnic US cohort (LUMINA L)Annals Of The Rheumatic Diseases, 2007
- Very low blood hydroxychloroquine concentration as an objective marker of poor adherence to treatment of systemic lupus erythematosusAnnals Of The Rheumatic Diseases, 2007
- Low blood concentration of hydroxychloroquine is a marker for and predictor of disease exacerbations in patients with systemic lupus erythematosusArthritis & Rheumatism, 2006
- Hydroxychloroquine concentration–response relationships in patients with rheumatoid arthritisArthritis & Rheumatism, 2002
- Influence of hydroxychloroquine on the bioavailability of oral metoprololBritish Journal of Clinical Pharmacology, 2000
- A Randomized Study of the Effect of Withdrawing Hydroxychloroquine Sulfate in Systemic Lupus ErythematosusThe New England Journal of Medicine, 1991
- Comparison of Several Regression Procedures for Method Comparison Studies and Determination of Sample Sizes Application of linear regression procedures for method comparison studies in Clinical Chemistry, Part IIcclm, 1984