Expression of CD90 on keratinocyte stem/progenitor cells
- 24 March 2006
- journal article
- Published by Oxford University Press (OUP) in British Journal of Dermatology
- Vol. 154 (6), 1062-1070
- https://doi.org/10.1111/j.1365-2133.2006.07209.x
Abstract
The identification and purification of keratinocyte stem cells (KSCs) that are capable of self-renewal and maintenance of differentiating cell populations could contribute both to our understanding of the biology of these cells, and to significant clinical applications, such as the culturing of keratinocytes for transplantation to severe burn wounds. Here, we report the detection of CD90(+) cells in cultured normal human epidermal keratinocytes and adult skin. To investigate the biological function of CD90(+) and CD90(-) keratinocytes. CD90(+) and CD90(-) keratinocytes were purified from adult skin and cultured keratinocytes using fluorescent activated cell sorting, and their biological abilities were analysed using both in vitro and in vivo assays. Flow cytometry (FCM) analysis identified approximately 18% of post-primary neonatal keratinocytes as CD90(+). However, during expansion of the culture, the expression level of CD90 rapidly decreased to about 2.5% at passage 10, while most of the keratinocytes maintained expression of alpha6 integrin. Purified CD90(+) keratinocytes demonstrated a sixfold higher cell growth rate than CD90(-) cells and the ability to form large (over 3 mm in diameter) colonies. We then quantitatively evaluated both populations using a previously described in vivo human epidermal cyst formation assay. Enhanced green fluorescent protein (EGFP)-labelled CD90(+) or CD90(-) keratinocytes were subcutaneously injected into nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. Six weeks after transplantation, EGFP(+) cell clusters in human epidermal cysts were evaluated using image analysis software. EGFP(+) cell cluster areas in the basal layer, derived from EGFP(+) CD90(+) cells, were eightfold larger than clusters of EGFP(+) CD90(-) cells. Furthermore, immunohistochemical staining and FCM analysis indicated that CD90 was expressed in most of the basal layer of the normal human epidermis. These results indicated that CD90 is a useful marker for the detection of human KSC-enriched populations in cultured human keratinocytes.This publication has 32 references indexed in Scilit:
- Isolation and characterization of cells with neurogenic potential from adult skeletal muscleBiochemical and Biophysical Research Communications, 2004
- CD90/Thy‐1 is preferentially expressed on blast cells of high risk acute myeloid leukaemias*British Journal of Haematology, 2004
- Isolation and characterization of neural progenitor cells from post‐mortem human cortexJournal of Neuroscience Research, 2003
- The long-term repopulating subset of hematopoietic stem cells is deterministic and isolatable by phenotypeImmunity, 1994
- Searching for hematopoietic stem cells: evidence that Thy-1.1lo Lin- Sca-1+ cells are the only stem cells in C57BL/Ka-Thy-1.1 bone marrow.The Journal of Experimental Medicine, 1992
- Three clonal types of keratinocyte with different capacities for multiplication.Proceedings of the National Academy of Sciences of the United States of America, 1987
- Immunology: Immunoglobulin-related domains for cell surface recognitionNature, 1985
- In Vitro Assessment of Keratinocyte AgingJournal of Investigative Dermatology, 1983
- A new way to evaluate the germinative compartment in human epidermis, using [3H]thymidine incorporation and immunoperoxidase staining of 67 K polypeptideBritish Journal of Dermatology, 1983
- A comparison of cell replacement in bone marrow, testis and three regions of surface epitheliumBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1979