Hydrolysis of lignocellulosics at low enzyme levels: Application of the AFEX process
- 1 April 1996
- journal article
- Published by Elsevier BV in Bioresource Technology
- Vol. 56 (1), 111-116
- https://doi.org/10.1016/0960-8524(95)00183-2
Abstract
The presumed high cost of cellulase enzymes is seen as a major economic hurdle in ethanol fuel production from lignocellulosic biomass. One way to reduce enzyme cost is to use much less enzyme per unit of biomass hydrolysed. The ammonia fiber explosion (AFEX) process produces a highly reactive biomass but the relationship between AFEX treatment conditions, enzyme loadings and hydrolysis yields has never been thoroughly explored. We report here the effects of AFEX treatment on the initial rates and 24 h hydrolysis yields for several crop residues and lignocellulosic materials using low enzyme levels (1–5 IU/g dry biomass). Near theoretical sugar yields are obtained for some AFEX treatment conditions at 1 IU/g for corn fiber and 5 IU/g for switchgrass. Implications of the experimental results are discussed.Keywords
This publication has 10 references indexed in Scilit:
- Ammonia-recycled percolation process for pretreatment of biomass feedstockApplied Biochemistry and Biotechnology, 1995
- Ethanol production from enzymatic hydrolysates of AFEX-treated coastal bermudagrass and switchgrassApplied Biochemistry and Biotechnology, 1995
- Processing and economic impacts of biomass delignification for ethanol productionApplied Biochemistry and Biotechnology, 1995
- Limiting factors in the simultaneous saccharification and fermentation process for conversion of cellulosic biomass to fuel ethanolApplied Biochemistry and Biotechnology, 1995
- Common aspects of acid prehydrolysis and steam explosion for pretreating woodBioresource Technology, 1991
- The effect of enzyme and substrate levels on the specific hydrolysis rate of pretreated poplar woodApplied Biochemistry and Biotechnology, 1991
- The ammonia freeze explosion (AFEX) processApplied Biochemistry and Biotechnology, 1991
- Effect of pretreatment on simultaneous saccharification and fermentation of hardwood into Acetone/ButanolApplied Biochemistry and Biotechnology, 1991
- The Effect of Pore Size Distribution on the Rate of Enzymatic Hydrolysis of Cellulosic SubstratesBio/Technology, 1985
- Use of Dinitrosalicylic Acid Reagent for Determination of Reducing SugarAnalytical Chemistry, 1959