Gel permeation chromatographic characterization of sodium hyaluronate and its fractions prepared by ultrasonic degradation
- 1 August 1990
- journal article
- Published by Springer Science and Business Media LLC in Chromatographia
- Vol. 30 (3), 201-204
- https://doi.org/10.1007/bf02274547
Abstract
High-molecular-weight sodium hyaluronate isolated from rooster combs was degraded by ultrasonication. The molecular weight of hyaluronate and its polydispersity was determined by gel-permeation chromatography. During 75-min treatment the molecular weight value decreased from 1.39×106 Da to 2.25×105 Da while the polydispersity of the molecular weight increased from 1.29 to 2.36. The reciprocal value of the square of the hyaluronate's molecular weight was linearly proportional to the time of ultrasonication.Keywords
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