Further Studies of the Role of Cyclic β-Glucans in Symbiosis. An ndvC Mutant of Bradyrhizobium japonicumSynthesizes Cyclodecakis-(1→3)-β-Glucosyl1
- 1 March 1999
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 119 (3), 1057-1064
- https://doi.org/10.1104/pp.119.3.1057
Abstract
The cyclic β-(1→3),β-(1→6)-d-glucan synthesis locus ofBradyrhizobium japonicum is composed of at least two genes, ndvB and ndvC. Mutation in either gene affects glucan synthesis, as well as the ability of the bacterium to establish a successful symbiotic interaction with the legume host soybean (Glycine max). B. japonicumstrain AB-14 (ndvB::Tn5) does not synthesize β-glucans, and strain AB-1 (ndvC::Tn5) synthesizes a cyclic β-glucan lacking β-(1→6)-glycosidic bonds. We determined that the structure of the glucan synthesized by strain AB-1 is cyclodecakis-(1→3)-β-d-glucosyl, a cyclic β-(1→3)-linked decasaccharide in which one of the residues is substituted in the 6 position with β-laminaribiose. Cyclodecakis-(1→3)-β-d-glucosyl did not suppress the fungal β-glucan-induced plant defense response in soybean cotyledons and had much lower affinity for the putative membrane receptor protein than cyclic β-(1→3),β-(1→6)-glucans produced by wild-typeB. japonicum. This is consistent with the hypothesis presented previously that the wild-type cyclic β-glucans may function as suppressors of a host defense response.Keywords
This publication has 49 references indexed in Scilit:
- Chalcone Synthase Transcripts Are Detected in Alfalfa Root Hairs Following Inoculation with Wild-TypeRhizobium melilotiMolecular Plant-Microbe Interactions®, 1997
- One‐step purification of the β‐glucan elicitor‐binding protein from soybean (Glycine max L.) roots and characterization of an anti‐peptide antiserumFEBS Letters, 1996
- Nodule Development Induced by Mutants ofBradyrhizobium japonicumDefective in Cyclic B-glucan SynthesisMolecular Plant-Microbe Interactions®, 1996
- Site-Directed Mutagenesis of the Beta-(13), Beta-(16)-D-Glucan Synthesis Locus ofBradyrhizobium japonicumMolecular Plant-Microbe Interactions®, 1995
- Enzymically Produced Cyclic α‐1,3‐Linked and α‐1,6‐Linked Oligosaccharides of d‐GlucoseEuropean Journal of Biochemistry, 1994
- Identification and cloning of a cyclic β-(1 → 3) β-(1 → 6)-d-glucan synthesis locus fromBradyrhizobium japonicumFEMS Microbiology Letters, 1993
- The presence of a novel type of surface polysaccharide in Rhizobium meliloti requires a new fatty acid synthase‐like gene cluster involved in symbiotic nodule developmentMolecular Microbiology, 1993
- Isolation and characterization of an ndvB locus from Rhizobium frediiMolecular Microbiology, 1992
- High‐affinity binding of a synthetic heptaglucoside and fungal glucan phytoalexin elicitors to soybean membranesFEBS Letters, 1990
- Solubilization of soybean membrane binding sites for fungal β‐glucans that elicit phytoalexin accumulationFEBS Letters, 1990