Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones
Open Access
- 12 April 2018
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 19 (4), 1172
- https://doi.org/10.3390/ijms19041172
Abstract
Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity. Enzymatically removing PNNs successfully enhances plasticity and thus functional recovery, particularly in spinal cord injury models. While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown. We aim to investigate the populations of PNN neurones involved in this functional motor recovery. Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns). CSPG-positive PNNs surrounded ~70–80% of Mns. Using WFA, only ~60% of the CSPG-positive PNNs co-localised with WFA in the spinal Mns, while ~15–30% of Mns showed CSPG-positive but WFA-negative PNNs. Selective labelling revealed that aggrecan encircled ~90% of alpha Mns. The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex. Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.Keywords
This publication has 60 references indexed in Scilit:
- Very long-term memories may be stored in the pattern of holes in the perineuronal netProceedings of the National Academy of Sciences of the United States of America, 2013
- Perineuronal nets protect fast-spiking interneurons against oxidative stressProceedings of the National Academy of Sciences of the United States of America, 2013
- Whole Animal Perfusion Fixation for RodentsJournal of Visualized Experiments, 2012
- Fiji: an open-source platform for biological-image analysisNature Methods, 2012
- Animals lacking link protein have attenuated perineuronal nets and persistent plasticityBrain, 2010
- Gamma and alpha motor neurons distinguished by expression of transcription factor Err3Proceedings of the National Academy of Sciences of the United States of America, 2009
- Versican V2 Assembles the Extracellular Matrix Surrounding the Nodes of Ranvier in the CNSJournal of Neuroscience, 2009
- Gamma motor neurons express distinct genetic markers at birth and require muscle spindle-derived GDNF for postnatal survivalNeural Development, 2009
- Chondroitinase ABC Promotes Sprouting of Intact and Injured Spinal Systems after Spinal Cord InjuryJournal of Neuroscience, 2006
- Differential expression of several molecules of the extracellular matrix in functionally and developmentally distinct regions of rat spinal cordCell and tissue research, 2006