Unsaturated phosphatidylcholine and its application in surgical adhesion
- 9 December 2005
- journal article
- Published by Wiley in Anz Journal of Surgery
- Vol. 75 (12), 1111-1114
- https://doi.org/10.1111/j.1445-2197.2005.03619.x
Abstract
It has been confirmed that surface-active phospholipid (SAPL), or surfactant, lines the surface of peritoneum and serves as a release and lubricating agent. The most important component in SAPL is phosphatidylcholine. A previous animal study showed that a saturated phosphatidylcholine, dipalmitoyl-phosphatidylcholine, reduced the formation of surgical adhesion. Latest studies have indicated that the dominant SAPL species at some sites outside the lung are not saturated phosphatidylcholine but, rather, are unsaturated phosphatidylcholine. High performance liquid chromatography was used to analyse the phosphatidylcholine profile of dialysate samples obtained from peritoneal dialysis patients. Friction tests were performed on dipalmitoyl-phosphatidylcholine and selected unsaturated phosphatidylcholine. It was discovered that unsaturated phosphatidylcholine was the dominant SAPL species inside the peritoneal cavity. They are palmitoyl-linoleoyl-phosphatidylcholine, palmitoyl-oleoylphosphatidylcholine and stearoylarachidonoylphosphatidylcholine. Most interestingly, there was no dipalmitoyl-phosphatidylcholine detected from these dialysate samples. The coefficients of static and dynamic friction from palmitoyllinoleoyl-phosphatidylcholine and palmitoyloleoyl-phosphatidylcholine were measured and found to be lower than that of dipalmitoyl-phosphatidylcholine. The results from the current study reveal that unsaturated phosphatidylcholine is the endogenous species inside the peritoneal cavity. This discovery offers further evidence that the dominant SAPL species at non-lung sites are unsaturated phosphatidylcholine, not saturated phosphatidylcholine, strongly indicating the difference between phosphatidylcholine species distribution at lung and non-lung sites. Unsaturated phosphatidylcholine has better anti-friction and lubrication properties than dipalmitoyl-phosphatidylcholine. Unsaturated phosphatidylcholine-based SAPL pharmaceutical products should be developed and evaluated.Keywords
This publication has 16 references indexed in Scilit:
- Eustachian tube surfactant is different from alveolar surfactant: determination of phospholipid composition of porcine eustachian tube lavage fluidJournal of Lipid Research, 2002
- Pulmonary and gastric surfactants. A comparison of the effect of surface requirements on function and phospholipid compositionComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2001
- SURGICAL ADHESIONS: EVIDENCE FOR ADSORPTION OF SURFACTANT TO PERITONEAL MESOTHELIUMAnz Journal of Surgery, 2000
- Composition of phospholipid classes and phosphatidylcholine molecular species of gastric mucosa and mucusBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1995
- High-Performance Liquid Chromatographic Analysis of Phospholipids from Different Sources with Combined Fluorescence and Ultraviolet DetectionAnalytical Biochemistry, 1994
- Phosphatidylcholine prevents postoperative peritoneal adhesions: An experimental study in the ratJournal of Surgical Research, 1991
- A comparison of the specificity of phosphatidylcholine synthesis by human fetal lung maintained in either organ or organotypic cultureBiochemical Journal, 1988
- DRY ARTIFICIAL LUNG SURFACTANT AND ITS EFFECT ON VERY PREMATURE BABIESThe Lancet, 1981
- Surface Tension Lowering Substance of the Canine Eustachian TubeAnnals of Otology, Rhinology & Laryngology, 1972
- Lubrication of synovial membrane.Annals Of The Rheumatic Diseases, 1971