Integrity of human sperm DNA assessed by the neutral comet assay and its relationship to semen parameters and clinical outcomes for the IVF-ET program
Open Access
- 1 January 2011
- journal article
- Published by The Korean Society for Reproductive Medicine in Clinical and Experimental Reproductive Medicine
- Vol. 38 (1), 10-17
- https://doi.org/10.5653/cerm.2011.38.1.10
Abstract
To explore potential relationships between sperm DNA integrity and both semen parameters and clinical outcomes. Semen analysis of 498 samples was performed according to the 2010 criteria of the World Health Organization. The sperm DNA fragmentation Index (DFI) of the semen samples was assessed using a neutral comet assay. Sperm DFI showed a significant correlation with semen parameters, including the patient's age, sperm viability, motility, morphology, and number of leukocytes (p<0.05). The sperm DFI values for asthenozoospermic (15.2%), oligoteratozoospermic (18.3%), asthenoteratozoospermic (17.5%), and oligoasthenoteratozoospermic semen samples (21.3%) were significantly higher than that observed in normozoospermic semen samples (10.5%, p<0.05). A sperm DFI value of 14% was used as a threshold of sperm DFI in assessing whether DNA was highly damaged. In 114 IVF-ET cycles, the fertilization rate of the sperm DFI <14% group (70 cycles, 61.7%) was significantly higher than that observed for the ≥14% group (44 cycles, 55.3%), but there was no difference in the other clinical outcomes between the two groups. In the ≥14% group, the pregnancy rates of the ICSI cycles (40.0%) and half-ICSI (44.0%) were higher than conventional IVF cycles (30.7%), but the difference was not statistically significant. Along with the conventional semen analysis, the sperm DFI assessed using the comet assay was shown to improve the quality of the semen evaluation. To evaluate the precise effect of ICSI on pregnancy rates in the patients who demonstrate high sperm DFI values, further study is necessary.Keywords
This publication has 40 references indexed in Scilit:
- Using the alkaline comet assay in prognostic tests for male infertility and assisted reproductive technology outcomesMutagenesis, 2008
- Semen DNA fragmentation index, evaluated with both TUNEL and Comet assay, and the ICSI outcome.2008
- Sperm DNA integrity assessment in prediction of assisted reproduction technology outcomeHuman Reproduction, 2006
- Effect of sperm DNA damage and sperm protamine deficiency on fertilization and embryo development post-ICSIReproductive BioMedicine Online, 2005
- Sperm chromatin structure assay (scsa®) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cyclesFertility and Sterility, 2004
- Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive techniqueHuman Reproduction, 2003
- Another Case of Imprinting Defect in a Girl with Angelman Syndrome Who Was Conceived by Intracytoplasmic Sperm InjectionAmerican Journal of Human Genetics, 2003
- Intracytoplasmic Sperm Injection May Increase the Risk of Imprinting DefectsAmerican Journal of Human Genetics, 2002
- Oxidative stress, DNA damage and the Y chromosomeReproduction, 2001
- Abnormal Sperm Parameters in Humans Are Indicative of an Abortive Apoptotic Mechanism Linked to the Fas-Mediated PathwayExperimental Cell Research, 1999