Differential effects of urethane and isoflurane on external urethral sphincter electromyography and cystometry in rats
- 1 October 2008
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Renal Physiology
- Vol. 295 (4), F1248-F1253
- https://doi.org/10.1152/ajprenal.90259.2008
Abstract
Urethane is a common and often preferred anesthetic agent for urodynamic recordings in rats, but its use is often restricted to terminal procedures because of a prolonged duration of action and potentially toxic effects. When urodynamic recordings are part of survival procedures in rodent experimental models, inhalation anesthetics, such as isoflurane, are frequently used and generally well tolerated. In this study, we compared the effects of urethane and isoflurane on lower urinary tract function. For this purpose, adult female rats were anesthetized by subcutaneous administration of urethane ( n = 6) or by inhalation of isoflurane ( n = 5). Micturition reflexes were assessed by concurrent cystometrogram and external urethral sphincter (EUS) electromyography (EMG) recordings to determine bladder contractile properties, EUS activation patterns, and the coordination between bladder contractions and EUS activation. Compared with urethane, isoflurane reduced frequency of bursts, firing frequency, and amplitude of EUS EMG activity during voiding as well as the EUS EMG amplitude during the bladder filling phase. Isoflurane also prolonged the bladder intercontractile intervals. Other several key functional aspects of the bladder contractile properties as well as the coordination between bladder contractions were not different between the two experimental groups. We conclude that micturition reflexes were differentially affected by isoflurane and urethane. Specifically, isoflurane exhibited a significant suppression of the EUS EMG activity and prolonged the bladder intercontractile intervals compared with urethane. We suggest that these anesthetic properties be taken into consideration during the experimental design and interpretation of urodynamic recordings in rodent models.Keywords
This publication has 28 references indexed in Scilit:
- Re-established micturition reflexes show differential activation patterns after lumbosacral ventral root avulsion injury and repair in ratsExperimental Neurology, 2008
- Functional characterization of PCFT/HCP1 as the molecular entity of the carrier-mediated intestinal folate transport system in the rat modelAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2008
- Role of pudendal afferents in voiding efficiency in the ratAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2008
- Serotonergic drugs and spinal cord transections indicate that different spinal circuits are involved in external urethral sphincter activity in ratsAmerican Journal of Physiology-Renal Physiology, 2007
- Effects of anesthesia on cystometry and leak point pressure of the female ratLife Sciences, 2001
- Isoflurane Depresses Motoneuron Excitability by a Direct Spinal ActionAnesthesia & Analgesia, 1999
- Isoflurane Depresses Motoneuron Excitability by a Direct Spinal ActionAnesthesia & Analgesia, 1999
- ABOLITION OF CYSTITIS-INDUCED BLADDER INSTABILITY BY LOCAL SPINAL CORD COOLINGJournal of Urology, 1998
- Suitability of urethane anesthesia for physiopharmacological investigations. Part 3: Other systems and conclusionsCellular and Molecular Life Sciences, 1986
- The nonstop transvesical cystometrogram in urethane-anesthetized rats: A simple procedure for quantitative studies on the various phases of urinary bladder voiding cycleJournal of Pharmacological Methods, 1986