Accumulation of DNA, Nuclear and Mitochondrial Debris, and ROS at Sites of Age-Related Cortical Cataract in Mice

Abstract

Purpose. Lenses from young and old mice were analyzed by laser scanning confocal microscopy (LSCM) with vital dyes, to determine whether age-related subcapsular and cortical cataracts were linked to the failure of lens fiber cells to degrade nuclei, DNA, and mitochondria properly and whether they result in the overproduction of reactive oxygen species (ROS) at the same sites.