Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool
Open Access
- 7 March 2013
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Soft Matter
- Vol. 9 (29), 6752-6756
- https://doi.org/10.1039/c3sm00131h
Abstract
The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.This publication has 11 references indexed in Scilit:
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