Environmentally persistent free radicals amplify ultrafine particle mediated cellular oxidative stress and cytotoxicity
Open Access
- 17 April 2009
- journal article
- Published by Springer Science and Business Media LLC in Particle and Fibre Toxicology
- Vol. 6 (1), 11
- https://doi.org/10.1186/1743-8977-6-11
Abstract
Combustion generated particulate matter is deposited in the respiratory tract and pose a hazard to the lungs through their potential to cause oxidative stress and inflammation. We have previously shown that combustion of fuels and chlorinated hydrocarbons produce semiquinone-type radicals that are stabilized on particle surfaces (i.e. environmentally persistent free radicals; EPFRs). Because the composition and properties of actual combustion-generated particles are complex, heterogeneous in origin, and vary from day-to-day, we have chosen to use surrogate particle systems. In particular, we have chosen to use the radical of 2-monochlorophenol (MCP230) as the EPFR because we have previously shown that it forms a EPFR on Cu(II)O surfaces and catalyzes formation of PCDD/F. To understand the physicochemical properties responsible for the adverse pulmonary effects of combustion by-products, we have exposed human bronchial epithelial cells (BEAS-2B) to MCP230 or the CuO/silica substrate. Our general hypothesis was that the EPFR-containing particle would have greater toxicity than the substrate species.Keywords
This publication has 46 references indexed in Scilit:
- Adverse Cardiovascular Effects with Acute Particulate Matter and Ozone Exposures: Interstrain Variation in MiceEnvironmental Health Perspectives, 2008
- Inner-Sphere Mechanism for Molecular Oxygen Reduction Catalyzed by Copper Amine OxidasesJournal of the American Chemical Society, 2008
- Fertility and Markers of Male Reproductive Function in Inuit and European Populations Spanning Large Contrasts in Blood Levels of Persistent OrganochlorinesEnvironmental Health Perspectives, 2008
- Potential health impacts of heavy-metal exposure at the Tar Creek Superfund site, Ottawa County, OklahomaEnvironmental Geochemistry and Health, 2008
- The role of oxidative stress in ambient particulate matter-induced lung diseases and its implications in the toxicity of engineered nanoparticlesFree Radical Biology & Medicine, 2008
- Neurocognitive Changes among Elderly Exposed to PCBs/PCDFs in TaiwanEnvironmental Health Perspectives, 2008
- Suitability of Flow Cytometry for Estimating Bacterial Biovolume in Natural Plankton Samples: Comparison with Microscopy DataApplied and Environmental Microbiology, 2007
- Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathioneChemico-Biological Interactions, 2006
- Biomass burning — a review of organic tracers for smoke from incomplete combustionApplied Geochemistry, 2002
- The metal-mediated formation of hydroxyl radical by aqueous extracts of cigarette tarBiochemical and Biophysical Research Communications, 1985