Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro
Open Access
- 8 January 2013
- journal article
- Published by Spandidos Publications in Experimental and Therapeutic Medicine
- Vol. 5 (3), 707-710
- https://doi.org/10.3892/etm.2013.889
Abstract
Drug combination therapies are common practice in the treatment of cancer. Cisplatin is the most active chemotherapeutic agent for lung cancer treatment. Osthole is a natural compound extracted from a number of medicinal plants. To determine whether osthole enhances the anticancer effect of cisplatin in human lung cancer, we treated NCI-H460 cells with osthole alone or in combination with cisplatin and evaluated cell growth and apoptosis using 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry and fluorescence microscopy. The results showed that, in comparison with single agent treatment, the combination of osthole and cisplatin resulted in greater efficacy in growth inhibition and apoptosis induction. Western blot analysis revealed that the combination effect of osthole and cisplatin was due to regulation of the Bcl-2 family proteins. Findings of this investigation suggested that osthole combined with cisplatin is a potential clinical chemotherapeutic approach in human lung cancer.Keywords
This publication has 20 references indexed in Scilit:
- Growth Inhibition and Apoptosis Induced by Osthole, A Natural Coumarin, in Hepatocellular CarcinomaPLOS ONE, 2012
- Osthole Suppresses Hepatocyte Growth Factor (HGF)-Induced Epithelial-Mesenchymal Transition via Repression of the c-Met/Akt/mTOR Pathway in Human Breast Cancer CellsJournal of Agricultural and Food Chemistry, 2011
- Combination of Tetrandrine with cisplatin enhances cytotoxicity through growth suppression and apoptosis in ovarian cancer in vitro and in vivoCancer Letters, 2011
- Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathwayJournal of Experimental & Clinical Cancer Research, 2011
- Curcumin Enhances the Effect of Cisplatin in Suppression of Head and Neck Squamous Cell Carcinoma via Inhibition of IKKβ Protein of the NFκB PathwayMolecular Cancer Therapeutics, 2010
- Thymoquinone and cisplatin as a therapeutic combination in lung cancer: In vitro and in vivoJournal of Experimental & Clinical Cancer Research, 2010
- Arsenic trioxide exerts synergistic effects with cisplatin on non-small cell lung cancer cells via apoptosis inductionJournal of Experimental & Clinical Cancer Research, 2009
- Cancer Statistics, 2008CA: A Cancer Journal for Clinicians, 2008
- International comparisons of survival from lung cancer: pitfalls and warningsNature Clinical Practice Oncology, 2007
- Antitumor effects of Osthol from Cnidium monnieri: an in vitro and in vivo studyPhytotherapy Research, 2007