Cyclophilin A Renders Human Immunodeficiency Virus Type 1 Sensitive to Old World Monkey but Not Human TRIM5α Antiviral Activity
- 15 May 2006
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 80 (10), 4683-90
- https://doi.org/10.1128/jvi.80.10.4683-4690.2006
Abstract
TRIM5α is an important mediator of antiretroviral innate immunity influencing species-specific retroviral replication. Here we investigate the role of the peptidyl prolyl isomerase enzyme cyclophilin A in TRIM5α antiviral activity. Cyclophilin A is recruited into nascent human immunodeficiency virus type 1 (HIV-1) virions as well as incoming HIV-1 capsids, where it isomerizes an exposed proline residue. Here we show that cyclophilin A renders HIV-1 sensitive to restriction by TRIM5α in cells from Old World monkeys, African green monkey and rhesus macaque. Inhibition of cyclophilin A activity with cyclosporine A, or reducing cyclophilin A expression with small interfering RNA, rescues TRIM5α-restricted HIV-1 infectivity. The effect of cyclosporine A on HIV-1 infectivity is dependent on TRIM5α expression, and expression of simian TRIM5α in permissive feline cells renders them able to restrict HIV-1 in a cyclosporine A-sensitive way. We use an HIV-1 cyclophilin A binding mutant (CA G89V) to show that cyclophilin A has different roles in restriction by Old World monkey TRIM5α and owl monkey TRIM-Cyp. TRIM-Cyp, but not TRIM5α, recruits its tripartite motif to HIV-1 capsid via cyclophilin A and, therefore, HIV-1 G89V is insensitive to TRIM-Cyp but sensitive to TRIM5α. We propose that cyclophilin A isomerization of a proline residue in the TRIM5α sensitivity determinant of the HIV-1 capsid sensitizes it to restriction by Old World monkey TRIM5α. In humans, where HIV-1 has adapted to bypass TRIM5α activity, the effects of cyclosporine A are independent of TRIM5α. We speculate that cyclophilin A alters HIV-1 sensitivity to a TRIM5α-independent innate immune pathway in human cells.This publication has 49 references indexed in Scilit:
- Naturally Occurring Capsid Substitutions Render HIV-1 Cyclophilin A Independent in Human Cells and TRIM-cyclophilin-resistant in Owl Monkey CellsOnline Journal of Public Health Informatics, 2005
- Differential Restriction of Human Immunodeficiency Virus Type 2 and Simian Immunodeficiency Virus SIVmac by TRIM5α AllelesJournal of Virology, 2005
- Cyclophilin B Escorts the Hepatitis C Virus RNA Polymerase: A Viral Achilles Heel?Molecular Cell, 2005
- Activation of a Phytopathogenic Bacterial Effector Protein by a Eukaryotic CyclophilinScience, 2005
- Simian Immunodeficiency Virus Infection of ChimpanzeesJournal of Virology, 2005
- Catalysis and Binding of Cyclophilin A with Different HIV-1 Capsid ConstructsBiochemistry, 2004
- The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeysNature, 2004
- Structural characterization of a proline-driven conformational switch within the Itk SH2 domainNature Structural & Molecular Biology, 2002
- Opitz G/BBB syndrome, a defect of midline development, is due to mutations in a new RING finger gene on Xp22Nature Genetics, 1997
- Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexesCell, 1991