Three-Dimensional Particle Tracking via Bifocal Imaging
- 21 June 2007
- journal article
- review article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 7 (7), 2043-2045
- https://doi.org/10.1021/nl0709120
Abstract
We introduce a bifocal imaging method that enables three-dimensional (3D) tracking of both fluorescent and nonfluorescent particles. We accomplish this by simultaneously imaging a focused plane, for in-plane position (x,y), and a defocused plane, for out-of-plane position (z), of a molecule using a single CCD camera. We applied our method to several systems including in vivo melanosome tracking and phagocytosed fluorescent bead tracking. We have achieved 2−5 nm accuracy with a 2−50 ms time resolution.Keywords
This publication has 9 references indexed in Scilit:
- Nanometric three-dimensional tracking of individual quantum dots in cellsApplied Physics Letters, 2007
- Imaging Intracellular Fluorescent Proteins at Nanometer ResolutionScience, 2006
- Beyond Rayleigh's criterion: A resolution measure with application to single-molecule microscopyProceedings of the National Academy of Sciences of the United States of America, 2006
- Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through timeProceedings of the National Academy of Sciences of the United States of America, 2005
- Hither and yon: a review of bi-directional microtubule-based transportPhysical Biology, 2004
- Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm LocalizationScience, 2003
- Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imagingOptics Letters, 2003
- Precise Nanometer Localization Analysis for Individual Fluorescent ProbesBiophysical Journal, 2002
- Three-dimensional particle imaging with a single cameraExperiments in Fluids, 1992