Generation of stable concentration gradients in 2D and 3D environments using a microfluidic ladder chamber
- 25 May 2007
- journal article
- Published by Springer Science and Business Media LLC in Biomedical Microdevices
- Vol. 9 (5), 627-635
- https://doi.org/10.1007/s10544-007-9051-9
Abstract
We have developed a simple microfluidic device for generating stable concentration gradients in 2D and 3D environments. The device, termed the Ladder Chamber, uses a two-compartment diffusion system to generate steady state gradients across flow-free channels that connect the source and sink channels. To demonstrate the utility of the Ladder Chamber for cell migration, neutrophil chemotaxis was successfully observed in soluble chemoattractant (IL-8) gradient. The Ladder Chamber’s simple design and experimental implementation make it an attractive approach for investigating cell migration and other biological experiments in well-defined gradients in 2D surfaces as well as in 3D gels.Keywords
This publication has 45 references indexed in Scilit:
- A parallel-gradient microfluidic chamber for quantitative analysis of breast cancer cell chemotaxisBiomedical Microdevices, 2006
- Generation of Complex, Static Solution Gradients in Microfluidic ChannelsJournal of the American Chemical Society, 2006
- Generating controlled molecular gradients in 3D gelsBiotechnology & Bioengineering, 2005
- Effective neutrophil chemotaxis is strongly influenced by mean IL-8 concentrationBiochemical and Biophysical Research Communications, 2004
- Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated deviceNature Biotechnology, 2002
- Soft Lithography in Biology and BiochemistryAnnual Review of Biomedical Engineering, 2001
- Lamellipodia in invasionSeminars in Cancer Biology, 2001
- A Fibrin or Collagen Gel Assay for Tissue Cell Chemotaxis: Assessment of Fibroblast Chemotaxis to GRGDSPExperimental Cell Research, 1999
- Quantitative analysis of cell motility and chemotaxis in Dictyostelium discoideum by using an image processing system and a novel chemotaxis chamber providing stationary chemical gradients.The Journal of cell biology, 1989
- Physical Chemistry of MacromoleculesJournal of the Electrochemical Society, 1962