Prevalence, risk factors and molecular epidemiology of Brachyspira pilosicoli in humans on the island of Bali, Indonesia
- 1 April 2004
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 53 (4), 325-332
- https://doi.org/10.1099/jmm.0.05415-0
Abstract
The purpose of this study was to investigate the prevalence and epidemiology of the anaerobic intestinal spirochaete Brachyspira pilosicoli amongst Indonesians living in rural and urban settings on the island of Bali. Faecal samples (n = 992) were collected on two occasions, 4 months apart, from people living in four traditional farming villages, one peri-urban location and one urban area. Samples were cultured anaerobically on selective agar and intestinal spirochaete isolates were confirmed to be B. pilosicoli by using a species-specific PCR. Forty-eight of the 121 isolates obtained were typed by using PFGE. A questionnaire was administered to participants and analysed in order to identify potential risk factors for colonization. Overall prevalence of carriage on the two visits was 11.8 and 12.6 %, respectively. Prevalence at different locations varied from 3.3 to 23.4 %, with the highest prevalence occurring in the peri-urban location. Considerable strain diversity was found, with the 48 isolates being divided into 44 PFGE types. There was no significant association between colonization and ownership of animals, contact with animals, farming, age or gender. On the first visit, colonization was significantly more common in people who used well water compared to those who used tap water. On the second visit, colonization was significantly more common in people with wet faeces than in those with normal faeces.Keywords
This publication has 25 references indexed in Scilit:
- Evaluation of selective media for the isolation of Brachyspira aalborgi from human faecesJournal of Medical Microbiology, 2003
- Rapid detection and identification of Brachyspira aalborgi from rectal biopsies and faeces of a patientResearch in Microbiology, 2003
- Carriage of intestinal spirochaetes by humans: epidemiological data from Western AustraliaEpidemiology and Infection, 2001
- Canine Intestinal Spirochetes Consist of Serpulina pilosicoli and a Newly Identified Group Provisionally Designated “ Serpulina canis ” sp. novJournal of Clinical Microbiology, 1998
- Pulsed-field gel electrophoresis for sub-specific differentiation ofSerpulina pilosicoli(formerly ‘Anguillina coli’)FEMS Microbiology Letters, 1996
- Isolations of intestinal spirochaetes from the blood of human patientsJournal of Hospital Infection, 1995
- Intestinal spirochaetes in a Gulf Arab populationEpidemiology and Infection, 1990
- Rectal spirochaetosis in homosexual men: characterisation of the organism and pathophysiology.Sexually Transmitted Infections, 1986
- Spirochaetosis: a remediable cause of diarrhoea and rectal bleeding?BMJ, 1981
- Intestinal Spirochaetosis as a Cause of Longstanding DiarrhoeaUpsala Journal of Medical Sciences, 1977