Controlled Release of PDGF-bb by Coaxial Electrospun Dextran/Poly(L-lactide-co-ε-caprolactone) Fibers with an Ultrafine Core/Shell Structure
- 1 January 2010
- journal article
- Published by Informa UK Limited in Journal of Biomaterials Science, Polymer Edition
- Vol. 21 (6-7), 803-819
- https://doi.org/10.1163/156856209x445302
Abstract
Membranes composed of dextran (DEX) and poly(L-lactide-co-ε-caprolactone) (PLCL) as ultrafine core/shell fibers for loading platelet-derived growth factor-bb (PDGF-bb) were produced by coaxial electrospinning. The morphology and core/shell structure of the DEX/PLCL fibers containing PDGF-bb were investigated by scanning electron microscopy and transmission electron microscopy. The loading amount of PDGF-bb in the DEX/PLCL membrane prepared at 0.1 ml/h of the inner solution flow rate (DEX/PLCL-1P) was much lower than that in DEX/PLCL-2P and DEX/PLCL-3P obtained at 0.2 ml/h and 0.3 ml/h inner solution flow rate, respectively. All three membranes showed obvious burst release of PDGF-bb in the first 2 days, and then the release behaviors were smoother and steadier. They all kept a fibrous morphology during the whole release period of 28 days. Studies on adhesion, proliferation and morphology of the vascular smooth muscle cells on the fibrous membranes of DEX/PLCL-1P, DEX/PLCL-2P and DEX/PLCL-3P, as well as PLCL and DEX/PLCL suggested that DEX/PLCL membranes containing PDGF-bb protected in the core of ultrafine fibers could positively promote cell attachment, and their cell activities were significantly higher than those of PLCL and DEX/PLCL membranes without PDGF-bb. The DEX/PLCL-2P membrane with fine core/shell structure of fibers would be the best choices for further applications.Keywords
This publication has 31 references indexed in Scilit:
- Fabrication and properties of core‐shell structure P(LLA‐CL) nanofibers by coaxial electrospinningJournal of Applied Polymer Science, 2008
- Poly(glycerol sebacate) Nanofiber Scaffolds by Core/Shell ElectrospinningMacromolecular Bioscience, 2008
- Electrospun biodegradable nanofiber nonwovens for controlled release of proteinsJournal of Controlled Release, 2008
- Coaxially electrospun micro/nanofibrous poly(ε-caprolactone)/eggshell-protein scaffoldBioinspiration & Biomimetics, 2008
- Electrospun Chitosan‐Coated Fibers of Poly(L‐lactide) and Poly(L‐lactide)/Poly(ethylene glycol): Preparation and CharacterizationMacromolecular Bioscience, 2008
- Electrospinning of Highly Porous Scaffolds for Cartilage RegenerationBiomacromolecules, 2008
- Electrospun cellulose acetate fiber mats containing curcumin and release characteristic of the herbal substancePolymer, 2007
- Electrospinning of poly(vinyl pyrrolidone)–iodine complex and poly(ethylene oxide)/poly(vinyl pyrrolidone)–iodine complex – a prospective route to antimicrobial wound dressing materialsEuropean Polymer Journal, 2007
- Preparation of core/shell PVP/PLA ultrafine fibers by coaxial electrospinningJournal of Applied Polymer Science, 2006
- Electrospinning of Polymeric Nanofibers for Tissue Engineering Applications: A ReviewTissue Engineering, 2006