T Cells Help To Amplify Inflammatory Responses Induced bySalmonella entericaSerotype Typhimurium in the Intestinal Mucosa
- 1 May 2008
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 76 (5), 2008-2017
- https://doi.org/10.1128/iai.01691-07
Abstract
Salmonella entericaserotype Typhimurium causes an acute inflammatory reaction in the ceca of streptomycin-pretreated mice. We determined global changes in gene expression elicited by serotype Typhimurium in the cecal mucosa. The gene expression profile was dominated by T-cell-derived cytokines and genes whose expression is known to be induced by these cytokines. Markedly increased mRNA levels of genes encoding gamma interferon (IFN-γ), interleukin-22 (IL-22), and IL-17 were detected by quantitative real-time PCR. Furthermore, the mRNA levels of genes whose expression is induced by IFN-γ, IL-22, or IL-17, including genes encoding macrophage inflammatory protein 2 (MIP-2), inducible nitric oxide synthase (Nos2), lipocalin-2 (Lcn2), MIP-1α, MIP-1β, and keratinocyte-derived cytokine (KC), were also markedly increased. To assess the importance of T cells in orchestrating this proinflammatory gene expression profile, we depleted T cells by using a monoclonal antibody prior to investigating cecal inflammation caused by serotype Typhimurium in streptomycin-pretreated mice. Depletion of CD3+T cells resulted in a dramatic reduction in gross pathology, a significantly reduced recruitment of neutrophils, and a marked reduction in mRNA levels ofIfn-γ,Il-22, Il-17, Nos2, Lcn2, andKc. Our results suggest that T cells play an important role in amplifying inflammatory responses induced by serotype Typhimurium in the cecal mucosa.Keywords
This publication has 43 references indexed in Scilit:
- Injection of Flagellin into the Host Cell Cytosol by Salmonella enterica Serotype TyphimuriumJournal of Biological Chemistry, 2007
- The Capsule Encoding theviaBLocus Reduces Interleukin-17 Expression and Mucosal Innate Responses in the Bovine Intestinal Mucosa during Infection withSalmonella entericaSerotype TyphiInfection and Immunity, 2007
- Salmonella enterica Serovar Typhimurium Exploits Inflammation to Compete with the Intestinal MicrobiotaPLoS Biology, 2007
- The Interleukin-22/STAT3 Pathway Potentiates Expression of Inducible Nitric-oxide Synthase in Human Colon Carcinoma CellsPublished by Elsevier BV ,2007
- IL-23 mediates inflammatory responses to mucoidPseudomonas aeruginosalung infection in miceAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2007
- Interleukin (IL)-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptidesThe Journal of Experimental Medicine, 2006
- Divergent roles of IL-23 and IL-12 in host defense against Klebsiella pneumoniae The Journal of Experimental Medicine, 2005
- Cytokines link osteoblasts and inflammation: microarray analysis of interleukin-17- and TNF-α-induced genes in bone cellsJournal of Leukocyte Biology, 2004
- Human macrophage activation programs induced by bacterial pathogensProceedings of the National Academy of Sciences of the United States of America, 2002
- Requirement of CDC42 for Salmonella -Induced Cytoskeletal and Nuclear ResponsesScience, 1996